Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/Phd-LOTUS_5_Limkain_b1
Location 108376..109049 Replicon plasmid pTthSNM1-7b
Accession NZ_AP025604
Organism Thermus thermophilus strain SNM1-7

Toxin (Protein)


Gene name vapC Uniprot ID Q53W55
Locus tag MWM12_RS10975 Protein ID WP_011229190.1
Coordinates 108376..108636 (+) Length 87 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A3P4AVG9
Locus tag MWM12_RS10980 Protein ID WP_008631493.1
Coordinates 108657..109049 (+) Length 131 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM12_RS10945 (TthSNM17_21470) 104757..105926 + 1170 WP_011229191.1 cytochrome P450 -
MWM12_RS12735 (TthSNM17_21480) 105923..106621 + 699 WP_011174499.1 lycopene cyclase domain-containing protein -
MWM12_RS10965 (TthSNM17_21490) 106626..107303 + 678 WP_039457849.1 lysophospholipid acyltransferase family protein -
MWM12_RS10970 (TthSNM17_21500) 107300..108322 + 1023 WP_244345462.1 glycosyltransferase -
MWM12_RS10975 (TthSNM17_21510) 108376..108636 + 261 WP_011229190.1 type II toxin-antitoxin system Phd/YefM family antitoxin Toxin
MWM12_RS10980 (TthSNM17_21520) 108657..109049 + 393 WP_008631493.1 type II toxin-antitoxin system VapC family toxin Antitoxin
MWM12_RS10985 (TthSNM17_21530) 109147..110715 + 1569 WP_203972100.1 phytoene desaturase family protein -
MWM12_RS10990 (TthSNM17_21540) 110712..111710 + 999 WP_011229187.1 type 2 isopentenyl-diphosphate Delta-isomerase -
MWM12_RS10995 (TthSNM17_21550) 111720..112112 + 393 WP_244345465.1 nucleotidyltransferase domain-containing protein -
MWM12_RS11000 (TthSNM17_21560) 112143..113090 - 948 WP_024119841.1 DUF523 and DUF1722 domain-containing protein -
MWM12_RS11005 (TthSNM17_21570) 113087..113875 - 789 WP_244452407.1 tryptophan-rich sensory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..239363 239363


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(4-67)

Antitoxin

PIN domain

(2-121)


Sequences

Toxin        


Download         Length: 87 a.a.        Molecular weight: 9894.40 Da        Isoelectric Point: 8.5953

>T40451 WP_011229190.1 NZ_AP025604:108376-108636 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Download         Length: 261 bp

>T40451 NZ_AP025604:108376-108636 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Antitoxin


Download         Length: 131 a.a.        Molecular weight: 14234.65 Da        Isoelectric Point: 10.6447

>AT40451 WP_008631493.1 NZ_AP025604:108657-109049 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Download         Length: 393 bp

>AT40451 NZ_AP025604:108657-109049 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q53W55

Antitoxin

Source ID Structure
AlphaFold DB A0A3P4AVG9

References