Detailed information of TA system TA040448

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Phd-LOTUS_5_Limkain_b1
• Location: 1040218..1040891
• Replicon: chromosome
• Accession: NZ_AP025596
• Organism: Thermus thermophilus strain SNM1-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q53W55
• Locus tag: MWM06_RS05870
• Protein ID: WP_011229190.1
• Coordinates: 1040218..1040478 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MWM06_RS05875
• Protein ID: WP_244454956.1
• Coordinates: 1040499..1040891 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM06_RS05840 (TthSNM11_11690)	1036599..1037768	+	1170	WP_011229191.1	cytochrome P450	-
MWM06_RS12880 (TthSNM11_11700)	1037765..1038463	+	699	WP_011174499.1	lycopene cyclase domain-containing protein	-
MWM06_RS05860 (TthSNM11_11710)	1038468..1039145	+	678	WP_011174498.1	lysophospholipid acyltransferase family protein	-
MWM06_RS05865 (TthSNM11_11720)	1039142..1040164	+	1023	WP_244345462.1	glycosyltransferase	-
MWM06_RS05870 (TthSNM11_11730)	1040218..1040478	+	261	WP_011229190.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Toxin
MWM06_RS05875 (TthSNM11_11740)	1040499..1040891	+	393	WP_244454956.1	type II toxin-antitoxin system VapC family toxin	Antitoxin
MWM06_RS05880 (TthSNM11_11750)	1040989..1042557	+	1569	WP_244454957.1	phytoene desaturase family protein	-
MWM06_RS05885 (TthSNM11_11760)	1042554..1043552	+	999	WP_011229187.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
MWM06_RS05890 (TthSNM11_11770)	1043562..1043825	+	264	WP_244454958.1	nucleotidyltransferase domain-containing protein	-
MWM06_RS05895 (TthSNM11_11780)	1043856..1044803	-	948	WP_024119841.1	DUF523 and DUF1722 domain-containing protein	-
MWM06_RS05900 (TthSNM11_11790)	1044800..1045588	-	789	WP_024119840.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-67)

Antitoxin

• PIN domain (2-121)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9894.40 Da
• Isoelectric Point: 8.5953

>T40448 WP_011229190.1 NZ_AP025596:1040218-1040478 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Nucleotide

• Length: 261 bp

>T40448 NZ_AP025596:1040218-1040478 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAAGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14229.63 Da
• Isoelectric Point: 10.3415

>AT40448 WP_244454956.1 NZ_AP025596:1040499-1040891 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLHHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEEFRHVPVPLVNPWEG

Nucleotide

• Length: 393 bp

>AT40448 NZ_AP025596:1040499-1040891 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCACCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGAGTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Structures

Toxin

• AlphaFold DB: Q53W55