Detailed information of TA system TA040446

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 491832..492403
• Replicon: chromosome
• Accession: NZ_AP025590
• Organism: Actinomyces oris K20

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: AoKodu_RS02110
• Protein ID: WP_010614342.1
• Coordinates: 491832..492209 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: F2UXK9
• Locus tag: AoKodu_RS02115
• Protein ID: WP_003787711.1
• Coordinates: 492206..492403 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AoKodu_RS02090 (AoKodu_04250)	487054..488901	+	1848	WP_010614338.1	phosphoenolpyruvate carboxykinase (GTP)	-
AoKodu_RS02095 (AoKodu_04260)	489879..490133	+	255	WP_010614339.1	hypothetical protein	-
AoKodu_RS02100 (AoKodu_04270)	490108..491160	+	1053	WP_240309975.1	hypothetical protein	-
AoKodu_RS02105 (AoKodu_04280)	491148..491690	-	543	WP_240309976.1	hypothetical protein	-
AoKodu_RS02110 (AoKodu_04290)	491832..492209	-	378	WP_010614342.1	type II toxin-antitoxin system VapC family toxin	Toxin
AoKodu_RS02115 (AoKodu_04300)	492206..492403	-	198	WP_003787711.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
AoKodu_RS02120 (AoKodu_04310)	492659..494971	-	2313	WP_248406773.1	hypothetical protein	-
AoKodu_RS02125 (AoKodu_04320)	495137..496444	+	1308	WP_010614345.1	phosphoribosylamine--glycine ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-42)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13767.77 Da
• Isoelectric Point: 6.3274

>T40446 WP_010614342.1 NZ_AP025590:c492209-491832 [Actinomyces oris K20]
VILVDTSVWIDHFHHSDPVLVSLLHEDEIGSHPLVAEELAMGSLRARDDVLKHLAHLRQFPVLSHDELLTLVAAHVLWGR
GLSPVDAHLLGSVTLVPGARLWTRDKRLLRAAEERGVSFTEGTAG

Nucleotide

• Length: 378 bp

>T40446 NZ_AP025590:c492209-491832 [Actinomyces oris K20]
GTGATCCTTGTCGACACCTCGGTATGGATCGATCACTTCCACCACTCGGACCCAGTACTCGTCAGCCTGCTGCACGAGGA
CGAGATCGGATCCCATCCACTCGTGGCGGAGGAACTGGCGATGGGGTCACTCAGGGCCAGAGATGACGTTCTCAAGCACT
TAGCGCACCTCCGCCAGTTCCCGGTCCTCTCACATGACGAGCTACTGACTCTGGTCGCAGCCCATGTGCTGTGGGGCCGG
GGACTCAGCCCAGTCGATGCGCACCTGCTCGGCTCCGTCACGCTGGTCCCCGGCGCCCGCCTGTGGACACGCGACAAGCG
ATTGCTCCGCGCTGCCGAAGAGCGCGGAGTCTCCTTCACCGAAGGAACCGCCGGCTGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6949.80 Da
• Isoelectric Point: 11.5457

>AT40446 WP_003787711.1 NZ_AP025590:c492403-492206 [Actinomyces oris K20]
VRTTVTLSDDLIANAQELTGITERTELLRAGLETLVRVESARRLAALGGSDRTASAAPRRRSASQ

Nucleotide

• Length: 198 bp

>AT40446 NZ_AP025590:c492403-492206 [Actinomyces oris K20]
GTGCGAACCACCGTGACACTCAGTGATGACCTCATTGCCAACGCGCAAGAGCTCACAGGCATCACTGAGCGCACCGAGCT
GCTGCGGGCCGGCCTGGAGACCCTGGTCAGAGTGGAGAGCGCACGTCGCCTTGCGGCTCTCGGCGGCTCTGATCGGACGG
CAAGCGCCGCTCCACGACGTCGGAGCGCCAGCCAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1F1VW34