Detailed information of TA system TA040440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 2562547..2563087
• Replicon: chromosome
• Accession: NZ_AP025585
• Organism: Pyramidobacter piscolens strain CE91-St28

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D1Y1I5
• Locus tag: MWL86_RS12260
• Protein ID: WP_009163717.1
• Coordinates: 2562794..2563087 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D1Y1I6
• Locus tag: MWL86_RS12255
• Protein ID: WP_009163718.1
• Coordinates: 2562547..2562807 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWL86_RS12225 (CE91St28_24010)	2557874..2558683	+	810	WP_009164667.1	type I-E CRISPR-associated protein Cas5/CasD	-
MWL86_RS12230 (CE91St28_24020)	2558673..2559437	+	765	WP_244388782.1	type I-E CRISPR-associated protein Cas6/Cse3/CasE	-
MWL86_RS12235 (CE91St28_24030)	2559438..2560313	+	876	WP_009164665.1	type I-E CRISPR-associated endonuclease Cas1e	-
MWL86_RS12240 (CE91St28_24040)	2560314..2560661	+	348	WP_009164664.1	type I-E CRISPR-associated endoribonuclease Cas2e	-
MWL86_RS12245 (CE91St28_24050)	2561684..2561908	+	225	WP_009163720.1	hypothetical protein	-
MWL86_RS12250	2561971..2562234	+	264	WP_009163719.1	hypothetical protein	-
MWL86_RS12255 (CE91St28_24070)	2562547..2562807	+	261	WP_009163718.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MWL86_RS12260 (CE91St28_24080)	2562794..2563087	+	294	WP_009163717.1	type II toxin-antitoxin system YafQ family toxin	Toxin
MWL86_RS12265 (CE91St28_24110)	2563909..2564373	+	465	WP_050768840.1	hypothetical protein	-
MWL86_RS12270 (CE91St28_24120)	2564500..2565393	+	894	WP_009163716.1	helix-turn-helix domain-containing protein	-
MWL86_RS12275 (CE91St28_24130)	2565570..2566595	+	1026	WP_009163715.1	dihydrodipicolinate reductase	-
MWL86_RS12280 (CE91St28_24140)	2567055..2567231	-	177	WP_009163714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-93)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11526.27 Da
• Isoelectric Point: 8.4955

>T40440 WP_009163717.1 NZ_AP025585:2562794-2563087 [Pyramidobacter piscolens]
MRTIKHSSAFKRDVKREKKGRYCEVFDTELLAIINNLANDVPLDEKYQDHELQRNLSGYRECHVRVDFLLLYQKIDGELK
ILRLERLGSHRQVLGIE

Nucleotide

• Length: 294 bp

>T40440 NZ_AP025585:2562794-2563087 [Pyramidobacter piscolens]
ATGCGGACGATTAAGCACTCTTCCGCTTTCAAACGCGACGTTAAACGCGAAAAGAAAGGACGCTACTGCGAAGTCTTCGA
TACCGAGCTGCTGGCGATCATCAACAATCTTGCGAACGATGTCCCCTTGGACGAGAAATATCAAGACCACGAACTGCAAC
GCAATCTCAGCGGATATCGCGAATGCCACGTGCGCGTCGACTTTCTGCTGCTGTACCAGAAGATCGACGGTGAATTGAAA
ATCCTCCGCCTCGAACGCCTCGGTTCTCACCGCCAGGTCCTCGGAATCGAATAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9647.89 Da
• Isoelectric Point: 5.0495

>AT40440 WP_009163718.1 NZ_AP025585:2562547-2562807 [Pyramidobacter piscolens]
MSTATINTRIDSNLKHQAEAIFSEIGLSTSQAIRLFYKQTVQSGGLPFQPDYNLPRETLEAMREAEEGKLTKITMDGLRA
MFHADD

Nucleotide

• Length: 261 bp

>AT40440 NZ_AP025585:2562547-2562807 [Pyramidobacter piscolens]
ATGTCGACCGCGACCATCAACACACGCATCGATTCGAATTTGAAACATCAAGCTGAAGCAATCTTCTCCGAGATCGGCCT
TTCCACCAGCCAGGCCATCCGACTCTTTTACAAACAGACCGTTCAATCTGGCGGCCTGCCCTTTCAGCCCGACTACAACT
TGCCCAGAGAGACCCTCGAAGCCATGAGAGAAGCCGAAGAAGGCAAACTGACGAAGATCACAATGGACGGACTGAGGGCA
ATGTTCCATGCGGACGATTAA

Structures

Toxin

• AlphaFold DB: D1Y1I5

Antitoxin

• AlphaFold DB: D1Y1I6