Detailed information of TA system TA040437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-DinJ
• Location: 1477028..1477593
• Replicon: chromosome
• Accession: NZ_AP025585
• Organism: Pyramidobacter piscolens strain CE91-St28

Toxin (Protein)

• Gene name: relE
• Uniprot ID: D1Y1L2
• Locus tag: MWL86_RS07015
• Protein ID: WP_009163744.1
• Coordinates: 1477303..1477593 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: D1Y1L3
• Locus tag: MWL86_RS07010
• Protein ID: WP_009163745.1
• Coordinates: 1477028..1477306 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWL86_RS06980 (CE91St28_13730)	1472414..1472851	+	438	WP_009163751.1	dUTP diphosphatase	-
MWL86_RS06985 (CE91St28_13740)	1472933..1474855	+	1923	WP_244388210.1	threonine--tRNA ligase	-
MWL86_RS06990 (CE91St28_13750)	1475142..1475450	+	309	WP_009163749.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
MWL86_RS06995 (CE91St28_13760)	1475447..1475791	+	345	WP_244388211.1	type II toxin-antitoxin system RelE/ParE family toxin	-
MWL86_RS07000 (CE91St28_13770)	1475808..1475960	+	153	WP_244388212.1	hypothetical protein	-
MWL86_RS07005 (CE91St28_13780)	1476276..1476620	+	345	WP_009163746.1	hypothetical protein	-
MWL86_RS07010 (CE91St28_13790)	1477028..1477306	+	279	WP_009163745.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
MWL86_RS07015 (CE91St28_13800)	1477303..1477593	+	291	WP_009163744.1	type II toxin-antitoxin system YafQ family toxin	Toxin
MWL86_RS07020 (CE91St28_13810)	1478076..1478594	+	519	WP_244388213.1	translation initiation factor IF-3	-
MWL86_RS07025 (CE91St28_13820)	1478622..1478822	+	201	WP_009163741.1	50S ribosomal protein L35	-
MWL86_RS07030 (CE91St28_13830)	1478850..1479206	+	357	WP_009163740.1	50S ribosomal protein L20	-
MWL86_RS07035 (CE91St28_13840)	1479345..1480388	+	1044	WP_244388214.1	phenylalanine--tRNA ligase subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (7-95)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11092.11 Da
• Isoelectric Point: 10.0372

>T40437 WP_009163744.1 NZ_AP025585:1477303-1477593 [Pyramidobacter piscolens]
VRQTKLTVKLTTAFKKDYKLAMKRGLKLELLDAVIEQLAMGKVLPKIYRDHELAGSWKGHRECHILSDWLLIYRIEESVL
VLTISRTGTHGDLFDK

Nucleotide

• Length: 291 bp

>T40437 NZ_AP025585:1477303-1477593 [Pyramidobacter piscolens]
GTGAGACAGACAAAGCTGACCGTCAAACTGACCACGGCATTTAAGAAGGACTACAAGCTCGCAATGAAGCGGGGATTAAA
ACTCGAACTTCTGGATGCCGTGATCGAACAGCTTGCCATGGGAAAGGTTCTGCCGAAGATCTACAGGGATCATGAGCTGG
CCGGAAGCTGGAAAGGACACAGGGAATGCCATATCCTCTCCGATTGGCTTTTGATCTATCGGATTGAAGAAAGCGTGCTG
GTGCTGACTATCTCCCGAACGGGAACGCACGGCGACCTGTTTGATAAATGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10176.64 Da
• Isoelectric Point: 4.5720

>AT40437 WP_009163745.1 NZ_AP025585:1477028-1477306 [Pyramidobacter piscolens]
MAGTTTNISIRMDSDLKAQADALFGELGMNLTTAFNIFVRQSLREGGIPFDICLHQPRQETIAAMLEAERIAKDPSVKGY
TDLDELFADLKK

Nucleotide

• Length: 279 bp

>AT40437 NZ_AP025585:1477028-1477306 [Pyramidobacter piscolens]
ATGGCTGGAACAACTACCAATATCAGCATCCGTATGGACAGTGACTTGAAAGCACAGGCAGACGCCTTATTCGGTGAGCT
TGGCATGAATCTTACGACGGCTTTCAACATCTTTGTGCGTCAGTCTCTCCGTGAGGGGGGGATTCCCTTTGATATCTGCC
TCCATCAGCCACGCCAGGAGACGATTGCCGCGATGCTGGAAGCGGAGAGGATTGCGAAAGACCCGTCAGTAAAGGGTTAT
ACCGACCTTGACGAGCTGTTCGCGGACCTTAAAAAGTGA

Structures

Toxin

• AlphaFold DB: D1Y1L2

Antitoxin

• AlphaFold DB: D1Y1L3