Detailed information of TA system TA040430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): FaRel2-ATfaRel2/-
• Location: 1277399..1278218
• Replicon: chromosome
• Accession: NZ_AP025566
• Organism: [Clostridium] hylemonae strain CE91-St63

Toxin (Protein)

• Gene name: faRel2
• Uniprot ID: C0BXT3
• Locus tag: MWL92_RS06025
• Protein ID: WP_006441953.1
• Coordinates: 1277399..1278013 (+)
• Length: 205 a.a.

Antitoxin (Protein)

• Gene name: ATfaRel2
• Uniprot ID: -
• Locus tag: MWL92_RS06030
• Protein ID: WP_244332579.1
• Coordinates: 1277994..1278218 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWL92_RS06015 (CE91St63_11680)	1272886..1274343	-	1458	WP_244332577.1	GntR family transcriptional regulator	-
MWL92_RS06020 (CE91St63_11690)	1274550..1277180	+	2631	WP_244332578.1	response regulator	-
MWL92_RS06025 (CE91St63_11700)	1277399..1278013	+	615	WP_006441953.1	hypothetical protein	Toxin
MWL92_RS06030 (CE91St63_11710)	1277994..1278218	+	225	WP_244332579.1	hypothetical protein	Antitoxin
MWL92_RS06035 (CE91St63_11720)	1278362..1280344	+	1983	WP_244332580.1	excinuclease ABC subunit UvrB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (65-169)

Antitoxin

• Protein of unknown function DUF6718 (1-74)

Sequences

Toxin

Protein

• Length: 205 a.a.
• Molecular weight: 24424.64 Da
• Isoelectric Point: 6.1570

>T40430 WP_006441953.1 NZ_AP025566:1277399-1278013 [[Clostridium] hylemonae]
MSDILQRDGLTIELLGRLSYKSNLGINLKKNLHYFDKEKLFEELIKVNEWYDSYDELYELALDYRIKSIQSAILKYERYY
PDHQTRKVFDDMLGFRSLCDNYEDVLTLKGIPELRVADMSKGKANDDGYRGIHVYFQLDGSHYPIEIQYNTYYDRQFNNW
LHKYVYKKKYDNSVGCHLRELYEGAKILTEKDFKEALEHVLFSS

Nucleotide

• Length: 615 bp

>T40430 NZ_AP025566:1277399-1278013 [[Clostridium] hylemonae]
ATGTCAGATATCTTACAAAGGGATGGTCTTACGATAGAATTGCTTGGACGCTTGTCTTATAAGTCAAATCTAGGAATTAA
CTTAAAGAAGAATTTACATTATTTTGATAAGGAAAAGCTTTTTGAAGAACTTATAAAGGTGAATGAGTGGTATGATTCGT
ATGATGAATTGTATGAATTAGCATTGGACTATAGAATTAAGAGTATTCAATCTGCAATATTGAAGTATGAAAGATATTAT
CCAGACCATCAGACCAGAAAAGTATTTGATGATATGTTAGGATTTCGTTCGCTATGTGATAATTACGAGGATGTGTTGAC
ATTGAAGGGAATTCCAGAGTTACGTGTGGCAGACATGTCAAAAGGAAAAGCAAATGATGATGGTTATAGAGGGATTCATG
TGTATTTTCAATTAGATGGCAGTCATTACCCTATCGAAATACAATATAATACATATTATGACAGGCAATTTAACAATTGG
CTTCATAAATATGTATATAAAAAGAAATATGATAATTCGGTAGGATGTCACTTGCGAGAATTATACGAAGGTGCTAAAAT
TCTAACAGAAAAAGATTTTAAGGAGGCGTTAGAACATGTGTTATTTAGTAGCTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8504.69 Da
• Isoelectric Point: 5.6112

>AT40430 WP_244332579.1 NZ_AP025566:1277994-1278218 [[Clostridium] hylemonae]
MCYLVAKHVGEVGSVALKTTHGKHLSEFKRSIEEKVGYEKIQLVTISRPSAYGEYEPYYFADTEEEFEKLVVNM

Nucleotide

• Length: 225 bp

>AT40430 NZ_AP025566:1277994-1278218 [[Clostridium] hylemonae]
ATGTGTTATTTAGTAGCTAAACATGTAGGTGAAGTCGGAAGTGTTGCATTAAAAACAACACATGGGAAACATCTTTCAGA
GTTTAAAAGATCAATAGAGGAAAAAGTTGGATATGAAAAAATTCAACTTGTTACTATCAGTAGACCTTCAGCTTATGGAG
AATATGAACCGTATTATTTTGCGGATACGGAGGAAGAATTTGAAAAACTTGTTGTAAATATGTAG

Structures

Toxin

• AlphaFold DB: C0BXT3