Detailed information of TA system TA040420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4322297..4323339
• Replicon: chromosome
• Accession: NZ_AP025556
• Organism: Delftia lacustris strain HQS1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: HQS1_RS19565
• Protein ID: WP_003109777.1
• Coordinates: 4322297..4322872 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: HQS1_RS19570
• Protein ID: WP_003050245.1
• Coordinates: 4322869..4323339 (-)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQS1_RS19540 (HQS1_38450)	4318735..4319460	-	726	WP_003050273.1	CBASS effector endonuclease NucC	-
HQS1_RS19545 (HQS1_38460)	4319499..4320401	-	903	WP_003105624.1	CBASS oligonucleotide cyclase	-
HQS1_RS19550 (HQS1_38470)	4320401..4320901	-	501	WP_003090159.1	type III CBASS phage resistance system CD-NTase-associated protein Cap7	-
HQS1_RS19555 (HQS1_38480)	4320898..4321368	-	471	WP_003105626.1	type III CBASS phage resistance system CD-NTase-associated protein Cap8	-
HQS1_RS19560 (HQS1_38490)	4321365..4322279	-	915	WP_003105629.1	AAA family ATPase	-
HQS1_RS19565 (HQS1_38500)	4322297..4322872	-	576	WP_003109777.1	PIN domain-containing protein	Toxin
HQS1_RS19570 (HQS1_38510)	4322869..4323339	-	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
HQS1_RS19575 (HQS1_38520)	4323543..4323926	+	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
HQS1_RS19580 (HQS1_38530)	4323923..4324156	+	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
HQS1_RS19585 (HQS1_38540)	4324173..4324532	+	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
HQS1_RS19590 (HQS1_38550)	4324544..4324942	+	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
HQS1_RS19595 (HQS1_38560)	4324939..4325631	+	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
HQS1_RS19600 (HQS1_38570)	4325628..4326539	+	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
HQS1_RS19605 (HQS1_38580)	4326529..4327947	+	1419	WP_020924934.1	TIGR03752 family integrating conjugative element protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4270151..4363673	93522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T40420 WP_003109777.1 NZ_AP025556:c4322872-4322297 [Delftia lacustris]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T40420 NZ_AP025556:c4322872-4322297 [Delftia lacustris]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT40420 WP_003050245.1 NZ_AP025556:c4323339-4322869 [Delftia lacustris]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT40420 NZ_AP025556:c4323339-4322869 [Delftia lacustris]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68