Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4219337..4219834 Replicon chromosome
Accession NZ_AP025551
Organism Escherichia coli strain LR-50

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE19_RS20580 Protein ID WP_001173343.1
Coordinates 4219337..4219510 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE19_RS20585 Protein ID WP_000398619.1
Coordinates 4219538..4219834 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE19_RS20570 (4216007) 4216007..4217464 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
QUE19_RS20575 (4217701) 4217701..4219218 + 1518 WP_001295074.1 lysine--tRNA ligase -
QUE19_RS20580 (4219337) 4219337..4219510 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE19_RS20585 (4219538) 4219538..4219834 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE19_RS20590 (4220061) 4220061..4220333 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QUE19_RS20595 (4220345) 4220345..4220575 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QUE19_RS20600 (4220756) 4220756..4222387 + 1632 WP_001216477.1 sensor histidine kinase -
QUE19_RS20605 (4222384) 4222384..4223103 + 720 WP_125090678.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40415 WP_001173343.1 NZ_AP025551:c4219510-4219337 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40415 NZ_AP025551:c4219510-4219337 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT40415 WP_000398619.1 NZ_AP025551:c4219834-4219538 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40415 NZ_AP025551:c4219834-4219538 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References