Detailed information of TA system TA040414

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 4123273..4123868
• Replicon: chromosome
• Accession: NZ_AP025551
• Organism: Escherichia coli strain LR-50

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: QUE19_RS20085
• Protein ID: WP_286330844.1
• Coordinates: 4123273..4123623 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: A0A240D6C8
• Locus tag: QUE19_RS20090
• Protein ID: WP_001223212.1
• Coordinates: 4123617..4123868 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE19_RS20065 (4118722)	4118722..4119744	-	1023	WP_001301928.1	ABC transporter permease	-
QUE19_RS20070 (4119758)	4119758..4121260	-	1503	WP_000205807.1	sugar ABC transporter ATP-binding protein	-
QUE19_RS20075 (4121400)	4121400..4122356	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
QUE19_RS20080 (4122666)	4122666..4123196	+	531	WP_000055070.1	inorganic diphosphatase	-
QUE19_RS20085 (4123273)	4123273..4123623	-	351	WP_286330844.1	endoribonuclease toxin ChpB	Toxin
QUE19_RS20090 (4123617)	4123617..4123868	-	252	WP_001223212.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
QUE19_RS20095 (4124080)	4124080..4124421	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
QUE19_RS20100 (4124424)	4124424..4128203	-	3780	WP_061359518.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12554.52 Da
• Isoelectric Point: 6.8700

>T40414 WP_286330844.1 NZ_AP025551:c4123623-4123273 [Escherichia coli]
MVKKSEFERGDIVLVGFYPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARQAKRIGLAADEVVEEALLRLQAVVK

Nucleotide

• Length: 351 bp

>T40414 NZ_AP025551:c4123623-4123273 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTTATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCAGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9301.61 Da
• Isoelectric Point: 4.6509

>AT40414 WP_001223212.1 NZ_AP025551:c4123868-4123617 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT40414 NZ_AP025551:c4123868-4123617 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCTACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A240D6C8