40413

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4031474..4032050 Replicon chromosome
Accession NZ_AP025551
Organism Escherichia coli strain LR-50

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QUE19_RS19650 Protein ID WP_001297643.1
Coordinates 4031763..4032050 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QUE19_RS19645 Protein ID WP_000063156.1
Coordinates 4031474..4031776 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE19_RS19630 (4028070) 4028070..4030220 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QUE19_RS19635 (4030270) 4030270..4030473 + 204 WP_000467859.1 YbdD/YjiX family protein -
QUE19_RS19640 (4030484) 4030484..4031440 + 957 WP_001297640.1 GTPase -
QUE19_RS19645 (4031474) 4031474..4031776 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QUE19_RS19650 (4031763) 4031763..4032050 - 288 WP_001297643.1 BrnT family toxin Toxin
QUE19_RS19655 (4032249) 4032249..4032946 - 698 Protein_3845 IS1 family transposase -
QUE19_RS19660 (4033003) 4033003..4033098 + 96 Protein_3846 dienelactone hydrolase family protein -
QUE19_RS19665 (4033244) 4033244..4034476 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QUE19_RS19670 (4034517) 4034517..4035797 + 1281 WP_232585742.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4032249..4032458 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T40413 WP_001297643.1 NZ_AP025551:c4032050-4031763 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T40413 NZ_AP025551:c4032050-4031763 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT40413 WP_000063156.1 NZ_AP025551:c4031776-4031474 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT40413 NZ_AP025551:c4031776-4031474 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References