Detailed information of TA system TA040395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 100365..100966
• Replicon: plasmid pLR-06-2
• Accession: NZ_AP025548
• Organism: Escherichia coli strain LR-06

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: QUE22_RS24440
• Protein ID: WP_024233187.1
• Coordinates: 100586..100966 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: QUE22_RS24435
• Protein ID: WP_001190712.1
• Coordinates: 100365..100586 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE22_RS24425 (EEGS01_47110)	97357..98628	-	1272	WP_286330328.1	restriction endonuclease subunit S	-
QUE22_RS24430 (EEGS01_47120)	98625..100182	-	1558	Protein_91	type I restriction-modification system subunit M	-
QUE22_RS24435 (EEGS01_47130)	100365..100586	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUE22_RS24440 (EEGS01_47140)	100586..100966	+	381	WP_024233187.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
QUE22_RS24445 (EEGS01_47150)	100971..101150	+	180	WP_000113018.1	hypothetical protein	-
QUE22_RS24450 (EEGS01_47160)	101178..102221	+	1044	WP_097475749.1	DUF968 domain-containing protein	-
QUE22_RS24455 (EEGS01_47170)	102310..102762	+	453	WP_286330329.1	Late promoter-activating protein	-
QUE22_RS24460 (EEGS01_47180)	102848..104041	+	1194	WP_068873707.1	terminase	-
QUE22_RS24465 (EEGS01_47190)	104041..104310	+	270	WP_223648222.1	large terminase	-
QUE22_RS24470 (EEGS01_47200)	104317..105525	+	1209	WP_286330330.1	terminase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib	-	1..145376	145376

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13574.27 Da
• Isoelectric Point: 5.1408

>T40395 WP_024233187.1 NZ_AP025548:100586-100966 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEDITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T40395 NZ_AP025548:100586-100966 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGATATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGAGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT40395 WP_001190712.1 NZ_AP025548:100365-100586 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT40395 NZ_AP025548:100365-100586 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6