Detailed information of TA system TA040390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2408877..2409515
• Replicon: chromosome
• Accession: NZ_AP025546
• Organism: Escherichia coli strain LR-06

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QUE22_RS11790
• Protein ID: WP_000813794.1
• Coordinates: 2409339..2409515 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QUE22_RS11785
• Protein ID: WP_001270286.1
• Coordinates: 2408877..2409293 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE22_RS11765 (2404029)	2404029..2404970	-	942	WP_001251313.1	ABC transporter permease	-
QUE22_RS11770 (2404971)	2404971..2405984	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
QUE22_RS11775 (2406002)	2406002..2407147	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
QUE22_RS11780 (2407392)	2407392..2408798	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
QUE22_RS11785 (2408877)	2408877..2409293	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QUE22_RS11790 (2409339)	2409339..2409515	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QUE22_RS11795 (2409737)	2409737..2409967	+	231	WP_000494244.1	YncJ family protein	-
QUE22_RS11800 (2410059)	2410059..2412020	-	1962	WP_094303868.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QUE22_RS11805 (2412093)	2412093..2412629	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
QUE22_RS11810 (2412721)	2412721..2413896	+	1176	WP_001236251.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2413936..2415201	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T40390 WP_000813794.1 NZ_AP025546:c2409515-2409339 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T40390 NZ_AP025546:c2409515-2409339 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT40390 WP_001270286.1 NZ_AP025546:c2409293-2408877 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT40390 NZ_AP025546:c2409293-2408877 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB