Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 824013..824667 Replicon chromosome
Accession NZ_AP025546
Organism Escherichia coli strain LR-06

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QUE22_RS04095 Protein ID WP_000244777.1
Coordinates 824260..824667 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE22_RS04090 Protein ID WP_000354046.1
Coordinates 824013..824279 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE22_RS04065 (819182) 819182..819925 + 744 WP_000951964.1 SDR family oxidoreductase -
QUE22_RS04070 (819982) 819982..821415 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
QUE22_RS04075 (821460) 821460..821771 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QUE22_RS04080 (821935) 821935..822594 + 660 WP_000250274.1 hemolysin III family protein -
QUE22_RS04085 (822790) 822790..823770 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QUE22_RS04090 (824013) 824013..824279 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE22_RS04095 (824260) 824260..824667 + 408 WP_000244777.1 protein YgfX Toxin
QUE22_RS04100 (824707) 824707..825228 - 522 WP_001055874.1 flavodoxin FldB -
QUE22_RS04105 (825340) 825340..826236 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE22_RS04110 (826261) 826261..826971 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE22_RS04115 (826977) 826977..828710 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T40378 WP_000244777.1 NZ_AP025546:824260-824667 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T40378 NZ_AP025546:824260-824667 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT40378 WP_000354046.1 NZ_AP025546:824013-824279 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT40378 NZ_AP025546:824013-824279 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References