Detailed information of TA system TA040373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 51793..52466
• Replicon: plasmid pHB8b
• Accession: NZ_AP025541
• Organism: Thermus thermophilus strain HB8_001

Toxin (Protein)

• Gene name: vapB
• Uniprot ID: Q53W54
• Locus tag: QUE39_RS10335
• Protein ID: WP_011229189.1
• Coordinates: 51793..52185 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapC
• Uniprot ID: Q53W55
• Locus tag: QUE39_RS10340
• Protein ID: WP_011229190.1
• Coordinates: 52206..52466 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE39_RS10310 (TthHB8_30490)	46967..47755	+	789	WP_011229185.1	hypothetical protein	-
QUE39_RS10315 (TthHB8_30500)	47752..48699	+	948	WP_011229186.1	DUF523 and DUF1722 domain-containing protein	-
QUE39_RS10320 (TthHB8_30510)	48730..49122	-	393	WP_011174492.1	nucleotidyltransferase domain-containing protein	-
QUE39_RS10325 (TthHB8_30520)	49132..50130	-	999	WP_011229187.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
QUE39_RS10330 (TthHB8_30530)	50127..51695	-	1569	WP_011229188.1	phytoene desaturase family protein	-
QUE39_RS10335 (TthHB8_30540)	51793..52185	-	393	WP_011229189.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUE39_RS10340 (TthHB8_30550)	52206..52466	-	261	WP_011229190.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUE39_RS10345 (TthHB8_30560)	52520..53542	-	1023	WP_011174497.1	glycosyltransferase	-
QUE39_RS10350 (TthHB8_30570)	53539..54216	-	678	WP_011174498.1	lysophospholipid acyltransferase family protein	-
QUE39_RS10355 (TthHB8_30580)	54221..54919	-	699	WP_011174499.1	lycopene cyclase domain-containing protein	-
QUE39_RS10360 (TthHB8_30590)	54916..56085	-	1170	WP_011229191.1	cytochrome P450	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..255953	255953

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-67)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14264.67 Da
• Isoelectric Point: 10.6447

>T40373 WP_011229189.1 NZ_AP025541:c52185-51793 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATTLRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 393 bp

>T40373 NZ_AP025541:c52185-51793 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAAGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTAGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGACCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9894.40 Da
• Isoelectric Point: 8.5953

>AT40373 WP_011229190.1 NZ_AP025541:c52466-52206 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Nucleotide

• Length: 261 bp

>AT40373 NZ_AP025541:c52466-52206 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Structures

Toxin

• AlphaFold DB: Q53W54

Antitoxin

• AlphaFold DB: Q53W55