Detailed information of TA system TA040368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 181514..182112
• Replicon: plasmid p20KCMC460_01
• Accession: NZ_AP025525
• Organism: Mycobacterium sp. 20KCMC460

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: MKCMC460_RS29945
• Protein ID: WP_238306246.1
• Coordinates: 181768..182112 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: MKCMC460_RS29940
• Protein ID: WP_238306245.1
• Coordinates: 181514..181774 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKCMC460_RS29915 (MKCMC460_60130)	176592..177233	+	642	WP_238306240.1	helix-turn-helix domain-containing protein	-
MKCMC460_RS29920 (MKCMC460_60140)	177368..177967	+	600	WP_238306241.1	hypothetical protein	-
MKCMC460_RS29925 (MKCMC460_60150)	178044..178304	+	261	WP_238306242.1	hypothetical protein	-
MKCMC460_RS29930 (MKCMC460_60170)	178607..178930	+	324	WP_238306243.1	hypothetical protein	-
MKCMC460_RS29935 (MKCMC460_60190)	180406..181248	+	843	WP_238306244.1	chemotaxis protein	-
MKCMC460_RS29940 (MKCMC460_60200)	181514..181774	+	261	WP_238306245.1	ribbon-helix-helix protein, CopG family	Antitoxin
MKCMC460_RS29945 (MKCMC460_60210)	181768..182112	+	345	WP_238306246.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MKCMC460_RS29950 (MKCMC460_60220)	182200..183516	+	1317	WP_186246668.1	IS256 family transposase	-
MKCMC460_RS31465 (MKCMC460_60230)	183649..184797	+	1149	WP_255736022.1	transglycosylase SLT domain-containing protein	-
MKCMC460_RS29960 (MKCMC460_60240)	184827..185318	+	492	WP_238306247.1	hypothetical protein	-
MKCMC460_RS31470 (MKCMC460_60250)	185401..186486	+	1086	WP_255736024.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	eccA5 / esxN / esxN / esxN / eccA5	1..380354	380354
	-	flank	IS/Tn	-	-	182200..183516	1316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-40)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12076.90 Da
• Isoelectric Point: 9.8887

>T40368 WP_238306246.1 NZ_AP025525:181768-182112 [Mycobacterium sp. 20KCMC460]
VVIGRAEIYWADLGVPSGSRPGKRRPVAVIQSDPYNASRLATVLAAVITSNTGLAAMPGNVFLPASTTGLSRDSVVNVTA
LVTLNKTDLSDRIGEIPPSLMHEVDRGLRRVLDL

Nucleotide

• Length: 345 bp

>T40368 NZ_AP025525:181768-182112 [Mycobacterium sp. 20KCMC460]
GTGGTGATCGGCCGCGCAGAGATCTACTGGGCCGACCTCGGCGTCCCCTCCGGCAGCCGACCCGGCAAGCGTCGTCCCGT
AGCAGTGATCCAATCCGATCCCTACAACGCCAGTCGCCTGGCCACGGTGCTCGCCGCCGTGATCACCTCCAACACGGGGC
TCGCAGCCATGCCAGGCAACGTGTTCCTACCTGCGTCCACAACGGGACTGTCACGTGATTCGGTCGTCAACGTCACGGCA
CTTGTGACGCTGAACAAGACCGACCTCAGCGATCGCATCGGGGAGATACCGCCGAGCTTGATGCACGAAGTCGACCGCGG
ACTTCGTCGCGTACTCGACCTTTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9524.37 Da
• Isoelectric Point: 4.2883

>AT40368 WP_238306245.1 NZ_AP025525:181514-181774 [Mycobacterium sp. 20KCMC460]
MKTAISLPDETFDRASRRARDLGMSRSEFFTRAAQRYLDELDSQSLTGQIDDALEHLGERLGDSDEGQAAAVAVGHRVLD
AADDQW

Nucleotide

• Length: 261 bp

>AT40368 NZ_AP025525:181514-181774 [Mycobacterium sp. 20KCMC460]
ATGAAGACCGCCATCTCCCTACCGGATGAGACGTTCGATCGCGCTTCGCGCCGCGCGCGTGACCTCGGCATGAGCCGGTC
GGAGTTCTTCACGCGGGCCGCACAGCGCTACCTCGACGAGTTGGACAGCCAATCGCTGACCGGGCAGATCGACGACGCTC
TCGAGCATCTTGGTGAGCGGCTTGGTGACAGCGATGAGGGGCAAGCCGCCGCGGTGGCCGTCGGGCACCGGGTGCTTGAC
GCCGCGGACGACCAGTGGTGA