Detailed information of TA system TA040366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 72647..73185
• Replicon: plasmid p20KCMC460_01
• Accession: NZ_AP025525
• Organism: Mycobacterium sp. 20KCMC460

Toxin (Protein)

• Gene name: relE
• Uniprot ID: M1FVK3
• Locus tag: MKCMC460_RS29400
• Protein ID: WP_015388906.1
• Coordinates: 72928..73185 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: M1FTM7
• Locus tag: MKCMC460_RS29395
• Protein ID: WP_007172404.1
• Coordinates: 72647..72931 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MKCMC460_RS29370	67785..68594	-	810	WP_238306179.1	hypothetical protein	-
MKCMC460_RS29375	68763..69116	+	354	WP_041321562.1	hypothetical protein	-
MKCMC460_RS29380 (MKCMC460_58960)	69518..70213	-	696	WP_238306180.1	DUF2637 domain-containing protein	-
MKCMC460_RS29385 (MKCMC460_58970)	71103..71798	+	696	WP_041321564.1	ParA family protein	-
MKCMC460_RS29390 (MKCMC460_58980)	71801..72565	+	765	WP_015388905.1	hypothetical protein	-
MKCMC460_RS29395 (MKCMC460_58990)	72647..72931	+	285	WP_007172404.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
MKCMC460_RS29400 (MKCMC460_59000)	72928..73185	+	258	WP_015388906.1	Txe/YoeB family addiction module toxin	Toxin
MKCMC460_RS29405 (MKCMC460_59010)	73163..73693	-	531	WP_015388907.1	hypothetical protein	-
MKCMC460_RS29410 (MKCMC460_59020)	73710..74744	-	1035	WP_084028306.1	hypothetical protein	-
MKCMC460_RS29415 (MKCMC460_59030)	75426..75995	+	570	WP_015388910.1	hypothetical protein	-
MKCMC460_RS29420 (MKCMC460_59040)	75997..77079	+	1083	WP_051197882.1	tyrosine-type recombinase/integrase	-
MKCMC460_RS29425 (MKCMC460_59050)	77228..77461	+	234	WP_081656402.1	hypothetical protein	-
MKCMC460_RS29430 (MKCMC460_59060)	77458..77721	+	264	WP_015388913.1	hypothetical protein	-
MKCMC460_RS29435 (MKCMC460_59070)	77824..78072	+	249	WP_015388914.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	eccA5 / esxN / esxN / esxN / eccA5	1..380354	380354

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (6-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-70)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 10133.52 Da
• Isoelectric Point: 9.8612

>T40366 WP_015388906.1 NZ_AP025525:72928-73185 [Mycobacterium sp. 20KCMC460]
VRSINFDPAAWEDFLFWLASDRKTARRITRLIAEIQRNPFAGIGKPEPLKGELSGYWSRRIDDEHRIVYRADDNEIKILK
ARYHY

Nucleotide

• Length: 258 bp

>T40366 NZ_AP025525:72928-73185 [Mycobacterium sp. 20KCMC460]
GTGAGAAGCATCAACTTCGATCCCGCCGCGTGGGAGGACTTTCTATTTTGGCTGGCTTCCGACCGCAAAACAGCACGGCG
CATCACCAGACTCATCGCAGAAATCCAGCGCAACCCGTTTGCTGGGATTGGAAAACCAGAACCGCTCAAGGGTGAACTAT
CCGGGTACTGGTCACGGCGCATCGACGACGAACACCGGATCGTCTACCGCGCAGACGATAACGAAATCAAGATCCTCAAA
GCCCGATACCACTACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10455.62 Da
• Isoelectric Point: 4.3885

>AT40366 WP_007172404.1 NZ_AP025525:72647-72931 [Mycobacterium sp. 20KCMC460]
MAISASEARQRLFPLLEQVNTDHEPVRITSKAGDAVLMSAADYDSWQETVYLLRSPENARRLMEAVARDNATRPTPGSYT
KSIDELEEMAGGQE

Nucleotide

• Length: 285 bp

>AT40366 NZ_AP025525:72647-72931 [Mycobacterium sp. 20KCMC460]
ATGGCTATCAGTGCAAGTGAGGCCCGGCAGCGCCTCTTTCCGCTGCTTGAACAAGTCAACACCGACCACGAGCCTGTGCG
GATCACGTCCAAGGCCGGAGATGCAGTGCTCATGTCGGCCGCTGACTACGATTCGTGGCAAGAAACGGTCTACCTCCTGC
GTTCCCCCGAGAATGCCCGCCGACTCATGGAGGCCGTCGCCCGAGACAACGCCACCCGGCCCACCCCCGGCAGCTACACC
AAGTCGATCGACGAACTCGAGGAGATGGCCGGCGGCCAGGAGTGA

Structures

Toxin

• AlphaFold DB: M1FVK3

Antitoxin

• AlphaFold DB: M1FTM7