Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-couple_hipB
Location 1889898..1891542 Replicon chromosome
Accession NZ_AP025519
Organism Pasteurella multocida strain Pm1

Toxin (Protein)


Gene name hipA Uniprot ID V4NI19
Locus tag MFE95_RS09415 Protein ID WP_005752891.1
Coordinates 1889898..1891205 (+) Length 436 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID V4NAB0
Locus tag MFE95_RS09420 Protein ID WP_005752888.1
Coordinates 1891207..1891542 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MFE95_RS09405 (PASm1_18460) 1885895..1888477 + 2583 WP_014326421.1 DNA mismatch repair protein MutS -
MFE95_RS09410 (PASm1_18470) 1888543..1889679 - 1137 WP_075266242.1 anhydro-N-acetylmuramic acid kinase -
MFE95_RS09415 (PASm1_18480) 1889898..1891205 + 1308 WP_005752891.1 HipA domain-containing protein Toxin
MFE95_RS09420 (PASm1_18490) 1891207..1891542 + 336 WP_005752888.1 helix-turn-helix transcriptional regulator Antitoxin
MFE95_RS09425 (PASm1_18500) 1891638..1892306 - 669 WP_014326419.1 nitroreductase family protein -
MFE95_RS09430 (PASm1_18510) 1892347..1893714 - 1368 WP_016504414.1 oxygen-independent coproporphyrinogen III oxidase -
MFE95_RS09435 (PASm1_18520) 1893738..1894166 - 429 WP_005719208.1 DUF2489 domain-containing protein -
MFE95_RS09440 (PASm1_18530) 1894172..1894741 - 570 WP_014326417.1 Der GTPase-activating protein YihI -
MFE95_RS09445 (PASm1_18540) 1894805..1895464 - 660 WP_005724855.1 bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(158-375)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-94)


Sequences

Toxin        


Download         Length: 436 a.a.        Molecular weight: 50526.31 Da        Isoelectric Point: 4.9118

>T40357 WP_005752891.1 NZ_AP025519:1889898-1891205 [Pasteurella multocida]
MEKLTLQIYLEQAWCDLAFLYFDSETYQLCNFEYLTDYVVQHYGKTGEHAVSLNYPVNVFIDDTTSLWSWLSDIAPAGAS
RRYWLEALDIKQESEEKQQFLLLKHAAISPIGNLRIKDAVPEQVEVRLFDLDKAKDRQSDFLEYVNQSGAMVGGATGAGG
EAPKLLLKRRENQVWIDNQQQGQDQDVSYLVKYPRGNYSPIDCDILRAEYHYYQELAQLGFDTIDTTRMRLEEGKRYPSL
WLPRFDIVQREGKTHRLAFESVYSLLQKQGGVLDHETTLRQLIERISTARLQFAQTDFVIEWVKRDLLNIAFGNSDNHGR
NMAFLRDEQRIWLAPIYDFAPMRADPEGIIRTTTWQTKGEYPQEFAGEYHFERIAEVLADLVEPTLLMQELRRLAGSLVD
LKSRLVQRNLPQSILDFPAIGFDYLPEKLKKWGLI

Download         Length: 1308 bp

>T40357 NZ_AP025519:1889898-1891205 [Pasteurella multocida]
ATGGAAAAACTTACACTGCAAATTTATCTTGAACAAGCTTGGTGCGATTTAGCTTTCTTGTATTTTGATTCAGAAACCTA
TCAACTGTGTAATTTCGAATACTTAACAGATTACGTGGTTCAGCACTATGGAAAAACGGGTGAACATGCTGTGTCGCTTA
ATTATCCAGTGAATGTTTTCATTGATGACACGACGTCGCTGTGGAGCTGGCTCAGTGATATCGCGCCTGCGGGAGCAAGT
CGTCGTTATTGGTTAGAAGCGTTAGACATTAAACAAGAATCAGAGGAAAAACAACAGTTTCTTTTACTGAAACATGCTGC
GATTTCCCCAATTGGCAATTTACGTATCAAAGATGCTGTGCCAGAACAAGTTGAAGTTCGCTTATTTGATCTAGACAAGG
CGAAAGATAGACAGAGTGATTTTTTAGAGTATGTGAACCAAAGTGGTGCGATGGTAGGAGGTGCGACAGGGGCTGGCGGC
GAAGCACCAAAATTGCTTCTTAAACGGCGAGAGAATCAAGTTTGGATTGATAACCAACAGCAAGGGCAGGATCAGGATGT
GAGTTATTTAGTGAAGTACCCACGTGGCAATTACTCACCCATTGATTGTGATATTTTACGTGCAGAATATCATTATTATC
AGGAACTTGCTCAACTGGGTTTTGACACAATAGATACGACTAGAATGAGGTTAGAAGAAGGAAAACGTTATCCTTCCCTA
TGGTTGCCTCGTTTTGATATTGTGCAACGAGAAGGAAAAACACATCGTCTTGCTTTTGAATCAGTGTATTCGCTATTACA
GAAACAAGGTGGTGTACTTGATCATGAAACGACACTTCGCCAGTTAATTGAGAGAATCTCGACGGCTCGTTTGCAGTTTG
CACAAACAGACTTCGTGATTGAATGGGTAAAGCGAGATTTGTTAAATATTGCCTTTGGCAATAGCGACAATCATGGTCGT
AATATGGCGTTTTTACGTGATGAACAACGTATTTGGCTCGCCCCAATTTATGATTTTGCGCCGATGCGTGCTGATCCAGA
AGGCATTATTCGTACGACTACTTGGCAAACTAAAGGGGAGTATCCACAGGAATTTGCTGGCGAGTATCACTTTGAGCGCA
TCGCAGAAGTATTAGCTGATTTAGTTGAACCAACGTTATTAATGCAAGAGTTACGCCGGTTAGCTGGCAGTTTAGTGGAT
TTAAAATCCCGATTAGTACAACGTAATCTACCTCAATCTATTTTAGATTTCCCTGCGATTGGTTTTGATTATTTACCTGA
AAAATTAAAAAAATGGGGGCTTATTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12520.60 Da        Isoelectric Point: 10.5259

>AT40357 WP_005752888.1 NZ_AP025519:1891207-1891542 [Pasteurella multocida]
MQITRRNASTKGASVAEIDRKKVRNEIIQAMLMGELSQGEALRKLRIEMFGINQQHYIQLVKVSRQTLSNIENDKGNYSV
ETINKVFKPFGLELGLVPVSKALLKELFSTA

Download         Length: 336 bp

>AT40357 NZ_AP025519:1891207-1891542 [Pasteurella multocida]
ATGCAAATCACTCGAAGAAATGCCTCTACCAAAGGGGCAAGTGTTGCGGAAATTGATCGTAAGAAAGTACGTAATGAAAT
CATTCAAGCGATGCTAATGGGGGAATTGAGCCAAGGTGAAGCATTGCGTAAATTGCGTATTGAAATGTTCGGTATTAATC
AACAACACTATATTCAGTTAGTAAAGGTATCACGCCAGACCCTATCGAATATTGAGAATGATAAGGGTAATTATAGTGTA
GAAACGATTAATAAAGTGTTTAAACCCTTTGGTCTGGAACTCGGCTTGGTACCTGTTTCTAAGGCATTATTAAAGGAACT
TTTTTCGACAGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V4NI19

Antitoxin

Source ID Structure
AlphaFold DB V4NAB0

References