Detailed information of TA system TA040354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 849868..850469
• Replicon: chromosome
• Accession: NZ_AP025519
• Organism: Pasteurella multocida strain Pm1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V4NB29
• Locus tag: MFE95_RS04225
• Protein ID: WP_016570073.1
• Coordinates: 850155..850469 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: MFE95_RS04220
• Protein ID: WP_016570074.1
• Coordinates: 849868..850158 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MFE95_RS04185 (PASm1_08120)	846190..847008	-	819	WP_015691057.1	hypothetical protein	-
MFE95_RS04190 (PASm1_08130)	847093..847353	-	261	WP_014391467.1	Rha family transcriptional regulator	-
MFE95_RS04195 (PASm1_08140)	847411..847863	-	453	WP_016570079.1	hypothetical protein	-
MFE95_RS04200 (PASm1_08150)	847912..848118	-	207	WP_016570078.1	helix-turn-helix transcriptional regulator	-
MFE95_RS04205 (PASm1_08160)	848249..848905	+	657	WP_016570077.1	XRE family transcriptional regulator	-
MFE95_RS04210 (PASm1_08170)	848973..849368	+	396	WP_016570076.1	hypothetical protein	-
MFE95_RS04215 (PASm1_08180)	849340..849843	+	504	WP_016570075.1	hypothetical protein	-
MFE95_RS04220 (PASm1_08190)	849868..850158	-	291	WP_016570074.1	putative addiction module antidote protein	Antitoxin
MFE95_RS04225 (PASm1_08200)	850155..850469	-	315	WP_016570073.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MFE95_RS04230 (PASm1_08210)	850732..850884	+	153	WP_197417893.1	hypothetical protein	-
MFE95_RS04235 (PASm1_08220)	851191..851481	+	291	WP_102827047.1	hypothetical protein	-
MFE95_RS04245 (PASm1_08230)	851977..852207	-	231	WP_223251317.1	hypothetical protein	-
MFE95_RS04250 (PASm1_08240)	852355..852639	+	285	WP_016570069.1	hypothetical protein	-
MFE95_RS04255 (PASm1_08250)	852626..852862	+	237	WP_016570068.1	hypothetical protein	-
MFE95_RS04260 (PASm1_08260)	852876..853037	+	162	WP_014390707.1	hypothetical protein	-
MFE95_RS04265 (PASm1_08270)	853040..854026	+	987	WP_016570067.1	hypothetical protein	-
MFE95_RS04270 (PASm1_08280)	854030..854881	+	852	WP_016533454.1	phage recombination protein Bet	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	813543..871880	58337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11714.64 Da
• Isoelectric Point: 9.3582

>T40354 WP_016570073.1 NZ_AP025519:c850469-850155 [Pasteurella multocida]
MLEIIETTIFNKWLKDLKDLSAKAAILARIGRAKNGNFGDHKSVGDGLYEMRIMKGAGYRVYYAQCKDVTYLLICGGDKS
TQKADIAKAKALWEEIKQKEELNV

Nucleotide

• Length: 315 bp

>T40354 NZ_AP025519:c850469-850155 [Pasteurella multocida]
ATGCTTGAGATAATTGAAACAACAATATTTAATAAATGGCTTAAAGATCTAAAAGATTTGTCGGCTAAAGCGGCTATTCT
TGCTCGTATTGGTCGGGCTAAAAATGGTAATTTTGGCGATCATAAATCAGTTGGTGATGGTTTATATGAAATGCGAATAA
TGAAAGGAGCTGGTTATAGGGTTTATTATGCACAATGTAAAGATGTAACCTATTTGCTAATTTGCGGGGGTGATAAATCT
ACACAAAAAGCAGATATCGCTAAAGCAAAAGCATTATGGGAAGAGATTAAGCAAAAAGAGGAGCTAAATGTATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10500.19 Da
• Isoelectric Point: 6.4688

>AT40354 WP_016570074.1 NZ_AP025519:c850158-849868 [Pasteurella multocida]
MSKKVELKPFDIAEYLDSEEMIAAYLSEVLETGGTNEFISALGDVARARGMTELAAKTGLGRESLYKTLSHGSKPRFDTI
MKIVQALGIKLVPTHI

Nucleotide

• Length: 291 bp

>AT40354 NZ_AP025519:c850158-849868 [Pasteurella multocida]
ATGAGTAAAAAAGTGGAATTAAAACCATTTGATATTGCTGAATACCTAGATAGTGAAGAAATGATTGCGGCATATTTAAG
TGAAGTTTTAGAAACAGGTGGCACTAATGAATTTATTTCTGCGCTAGGTGATGTCGCAAGAGCGAGAGGAATGACGGAGT
TAGCAGCAAAAACAGGATTAGGAAGAGAAAGTCTTTATAAAACATTATCACATGGAAGTAAGCCACGTTTTGATACTATA
ATGAAAATTGTGCAAGCATTAGGTATTAAACTTGTCCCAACGCATATCTAA

Structures

Toxin

• AlphaFold DB: V4NB29