Detailed information of TA system TA040335

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 456466..457265
• Replicon: chromosome
• Accession: NZ_AP025504
• Organism: Vibrio pelagius strain ATCC 25916

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: vsple_RS16205
• Protein ID: WP_261883860.1
• Coordinates: 456466..456999 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: vsple_RS16210
• Protein ID: WP_261883861.1
• Coordinates: 456996..457265 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
vsple_RS16185	452980..453153	+	174	WP_255232586.1	hypothetical protein	-
vsple_RS16190	453474..453794	+	321	WP_261883857.1	hypothetical protein	-
vsple_RS16195	453961..455220	+	1260	WP_261883858.1	hydroxymethylglutaryl-CoA reductase	-
vsple_RS16200	455254..456177	+	924	WP_261883859.1	LysR family transcriptional regulator	-
vsple_RS16205	456466..456999	-	534	WP_261883860.1	GNAT family N-acetyltransferase	Toxin
vsple_RS16210	456996..457265	-	270	WP_261883861.1	DUF1778 domain-containing protein	Antitoxin
vsple_RS16215	457328..457450	+	123	Protein_378	IS110 family transposase	-
vsple_RS16220	457670..457918	+	249	WP_261883862.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
vsple_RS16225	457908..458199	+	292	Protein_380	type II toxin-antitoxin system RelE/ParE family toxin	-
vsple_RS16230	458331..459176	-	846	WP_261883863.1	SH3 domain-containing protein	-
vsple_RS16235	459327..459911	-	585	WP_261883864.1	hypothetical protein	-
vsple_RS16240	460026..460649	-	624	WP_261883865.1	OST5 family protein	-
vsple_RS16245	460774..461751	-	978	WP_261883866.1	hypothetical protein	-
vsple_RS16250	461890..462024	-	135	WP_261883867.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-81)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20109.40 Da
• Isoelectric Point: 9.8580

>T40335 WP_261883860.1 NZ_AP025504:c456999-456466 [Vibrio pelagius]
VSWGKEFVELSKSKHDRNSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSSQPLLNQKFAICAFYSVAPSSISRETLPA
QQAKKLPRYPIPVFVLAQLAVHKEFHGSGLGKISLIRALKYLWEVNRHMRAYAIVVDCLTDSAQAFYAKFGFEVLCEHNG
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T40335 NZ_AP025504:c456999-456466 [Vibrio pelagius]
GTGAGTTGGGGTAAAGAGTTCGTAGAGCTTAGTAAATCAAAGCATGATCGCAACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTTCTCAAC
CACTGTTAAATCAAAAATTCGCTATTTGTGCATTTTATAGTGTTGCACCGAGTTCAATCAGCCGAGAAACATTACCAGCA
CAACAAGCAAAGAAACTTCCTAGGTATCCAATTCCTGTTTTTGTGTTGGCTCAGTTAGCTGTGCACAAAGAGTTTCATGG
CTCTGGGCTCGGAAAGATTAGTCTAATTCGAGCACTGAAGTACTTATGGGAAGTGAATCGCCATATGAGGGCGTACGCAA
TTGTTGTGGACTGTCTGACAGATTCCGCTCAAGCGTTCTATGCGAAGTTCGGCTTTGAAGTGCTGTGCGAACATAATGGG
CGTATTCGTATGTTTTTACCAATGAAAACGGTAGAACAGCTTTTTAATCAATAG

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9874.35 Da
• Isoelectric Point: 4.5837

>AT40335 WP_261883861.1 NZ_AP025504:c457265-456996 [Vibrio pelagius]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATRVIEEHESIMVKDSVFDEFMAACNKAKAPNQALLEAA
KFTDESGIK

Nucleotide

• Length: 270 bp

>AT40335 NZ_AP025504:c457265-456996 [Vibrio pelagius]
ATGGCAACCGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCCGAAAAAGCATCGGCTTTACTTGGTTT
AAAAAGTCTCACTGAATATGTAGTGAGACTAATGGACGAAGATGCGACTCGTGTTATTGAGGAACATGAAAGTATTATGG
TTAAAGACAGTGTCTTTGACGAGTTTATGGCTGCGTGCAATAAAGCAAAAGCACCGAATCAAGCTTTGCTTGAGGCTGCC
AAATTTACAGATGAGAGTGGTATTAAGTGA