Detailed information of TA system TA040281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 6114114..6114749
• Replicon: chromosome
• Accession: NZ_AP025343
• Organism: Paenibacillus azoreducens strain J34TS1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L6442_RS27205
• Protein ID: WP_127606057.1
• Coordinates: 6114114..6114464 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: L6442_RS27210
• Protein ID: WP_136606770.1
• Coordinates: 6114468..6114749 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6442_RS27200	6111607..6113829	-	2223	WP_212978912.1	alpha-galactosidase	-
L6442_RS27205	6114114..6114464	-	351	WP_127606057.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
L6442_RS27210	6114468..6114749	-	282	WP_136606770.1	ribbon-helix-helix protein, CopG family	Antitoxin
L6442_RS27215	6114983..6116176	-	1194	WP_212978911.1	alanine racemase	-
L6442_RS27220	6116595..6117692	-	1098	WP_194234475.1	DUF4367 domain-containing protein	-
L6442_RS27225	6117980..6119296	-	1317	WP_212978910.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12852.79 Da
• Isoelectric Point: 5.1284

>T40281 WP_127606057.1 NZ_AP025343:c6114464-6114114 [Paenibacillus azoreducens]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAASHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMKKVDDSLQISLSLIDF

Nucleotide

• Length: 351 bp

>T40281 NZ_AP025343:c6114464-6114114 [Paenibacillus azoreducens]
TTGATCGTAAAACGCGGCGACGTTTTTTTTGCGGATCTTTCACCCGTTGTCGGTTCTGAGCAAGGCGGAGTGAGACCGGT
ACTGATCATCCAGAATGATATCGGCAACCGCTTTAGTCCCACGGTCATCGTGGCGGCTATTACGGCCCAGATCCAAAAAG
CCAAGCTGCCGACGCATGTGGAAATCGATGCGGCTTCGCATGGCTTTGACAGGGACTCTGTTATATTGCTGGAGCAAATC
CGAACCATTGACAAGCAGAGGCTGACGGACAAAATTACCCATCTGGACGAAGAAACGATGAAAAAGGTGGATGATTCCTT
GCAGATCAGTCTCAGCTTGATCGACTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10711.36 Da
• Isoelectric Point: 7.3178

>AT40281 WP_136606770.1 NZ_AP025343:c6114749-6114468 [Paenibacillus azoreducens]
VANMQNTKRIMISLPDHLLQEVDGIVAMENSNRSELIRQAMKLYLHERKKRYIRESMQRGYMEMAKINLTMASEAFHAEE
DADSTLGRLVSGV

Nucleotide

• Length: 282 bp

>AT40281 NZ_AP025343:c6114749-6114468 [Paenibacillus azoreducens]
GTGGCTAATATGCAGAATACCAAACGAATTATGATCAGCTTGCCGGATCATCTCTTACAGGAAGTGGACGGTATCGTAGC
TATGGAGAATTCCAACCGCAGTGAATTGATCAGGCAGGCCATGAAGTTGTATTTGCATGAACGGAAAAAGCGTTATATCC
GGGAATCCATGCAGCGCGGTTATATGGAAATGGCCAAAATCAACTTGACCATGGCCTCTGAAGCGTTTCATGCGGAGGAA
GATGCAGACAGCACTCTAGGCCGCTTAGTAAGCGGGGTGTAA