Detailed information of TA system TA040280

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 3053086..3053651
• Replicon: chromosome
• Accession: NZ_AP025343
• Organism: Paenibacillus azoreducens strain J34TS1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: L6442_RS13315
• Protein ID: WP_194233053.1
• Coordinates: 3053310..3053651 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: L6442_RS13310
• Protein ID: WP_212977319.1
• Coordinates: 3053086..3053316 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6442_RS13285	3048527..3049228	-	702	WP_212977314.1	transglutaminase family protein	-
L6442_RS13290	3049272..3050048	-	777	WP_212977315.1	AraC family transcriptional regulator	-
L6442_RS13295	3050232..3050705	-	474	WP_212977316.1	hypothetical protein	-
L6442_RS13300	3050653..3051510	-	858	WP_212977317.1	hypothetical protein	-
L6442_RS13305	3051872..3052651	-	780	WP_212977318.1	zinc ribbon domain-containing protein	-
L6442_RS13310	3053086..3053316	+	231	WP_212977319.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
L6442_RS13315	3053310..3053651	+	342	WP_194233053.1	endoribonuclease MazF	Toxin
L6442_RS13320	3053758..3053976	-	219	WP_212977320.1	helix-turn-helix transcriptional regulator	-
L6442_RS13325	3054094..3054381	+	288	WP_212977321.1	hypothetical protein	-
L6442_RS13330	3054579..3055400	-	822	WP_212977322.1	alpha/beta fold hydrolase	-
L6442_RS13335	3055569..3056939	-	1371	WP_212977323.1	PLP-dependent aminotransferase family protein	-
L6442_RS13340	3057600..3057761	+	162	WP_212977324.1	thiazolylpeptide-type bacteriocin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3043114..3057761	14647

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-112)

Antitoxin

• Antitoxin PemI-like (2-76)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12513.56 Da
• Isoelectric Point: 5.8098

>T40280 WP_194233053.1 NZ_AP025343:3053310-3053651 [Paenibacillus azoreducens]
MVSYSYVPDRGDLVWLQFNPQAGHEQAGKRPALVISPAAYNGKVGLSLLCPVTSKIKGYPFEVVIPQDVPIEGVILSDQV
KSLDWQSRQATFICKVPDETLVEVVSKMELLIR

Nucleotide

• Length: 342 bp

>T40280 NZ_AP025343:3053310-3053651 [Paenibacillus azoreducens]
ATGGTAAGTTATTCGTATGTGCCAGATCGCGGCGACTTGGTGTGGTTGCAATTCAACCCACAAGCAGGTCATGAACAAGC
TGGAAAGCGTCCTGCCTTGGTCATCTCACCTGCCGCGTATAACGGTAAGGTTGGGCTTTCTCTGCTCTGTCCGGTAACGT
CCAAGATCAAGGGCTACCCATTCGAGGTTGTCATTCCGCAGGATGTACCTATCGAGGGGGTAATACTCTCCGATCAGGTA
AAAAGTCTCGACTGGCAGTCTAGACAGGCAACCTTCATCTGCAAAGTACCAGATGAGACGTTGGTTGAGGTTGTTTCCAA
AATGGAGTTGTTGATTCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8350.49 Da
• Isoelectric Point: 4.7469

>AT40280 WP_212977319.1 NZ_AP025343:3053086-3053316 [Paenibacillus azoreducens]
MFQVQKWGNSLGIRIPKSLALKVGIKEGSEVDLDVEDGHLVIKPKSATLDELLAQVTPENLHKEVSTGEPQGQESW

Nucleotide

• Length: 231 bp

>AT40280 NZ_AP025343:3053086-3053316 [Paenibacillus azoreducens]
ATGTTTCAAGTTCAAAAATGGGGTAACAGCTTAGGCATTCGCATCCCGAAGTCCCTTGCCTTGAAGGTTGGTATTAAGGA
AGGCTCTGAGGTCGATCTTGATGTGGAAGATGGTCATCTTGTAATAAAGCCGAAGTCGGCAACACTGGATGAACTTCTTG
CCCAGGTCACGCCGGAAAATCTCCATAAAGAGGTTTCTACTGGTGAACCACAAGGACAGGAGTCATGGTAA