Detailed information of TA system TA040278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 1884436..1885072
• Replicon: chromosome
• Accession: NZ_AP025342
• Organism: Bacillus paralicheniformis strain J41TS8

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M5P3Q9
• Locus tag: L6430_RS09440
• Protein ID: WP_003179128.1
• Coordinates: 1884722..1885072 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M5PDU2
• Locus tag: L6430_RS09435
• Protein ID: WP_006638778.1
• Coordinates: 1884436..1884717 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6430_RS09415	1879546..1881030	+	1485	WP_041817155.1	PH domain-containing protein	-
L6430_RS09420	1881027..1881626	-	600	WP_020450255.1	rhomboid family intramembrane serine protease	-
L6430_RS09425	1881970..1882929	+	960	WP_165395380.1	outer membrane lipoprotein carrier protein LolA	-
L6430_RS09430	1883155..1884324	+	1170	WP_041817156.1	alanine racemase	-
L6430_RS09435	1884436..1884717	+	282	WP_006638778.1	hypothetical protein	Antitoxin
L6430_RS09440	1884722..1885072	+	351	WP_003179128.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
L6430_RS09445	1885190..1886017	+	828	WP_065643252.1	RsbT co-antagonist protein RsbRA	-
L6430_RS09450	1886021..1886386	+	366	WP_065643251.1	STAS domain-containing protein	-
L6430_RS09455	1886389..1886790	+	402	WP_065643250.1	anti-sigma regulatory factor	-
L6430_RS09460	1886801..1887808	+	1008	WP_065643249.1	PP2C family protein-serine/threonine phosphatase	-
L6430_RS09465	1887866..1888192	+	327	WP_065643248.1	anti-sigma factor antagonist	-
L6430_RS09470	1888192..1888674	+	483	WP_065643247.1	anti-sigma B factor RsbW	-
L6430_RS09475	1888640..1889431	+	792	WP_065643260.1	RNA polymerase sigma factor SigB	-
L6430_RS09480	1889428..1890027	+	600	WP_065643246.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12990.03 Da
• Isoelectric Point: 5.7234

>T40278 WP_003179128.1 NZ_AP025342:1884722-1885072 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Nucleotide

• Length: 351 bp

>T40278 NZ_AP025342:1884722-1885072 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10649.03 Da
• Isoelectric Point: 4.6920

>AT40278 WP_006638778.1 NZ_AP025342:1884436-1884717 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT40278 NZ_AP025342:1884436-1884717 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6I7FHI4

Antitoxin

• AlphaFold DB: M5PDU2