Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 4093169..4093805 Replicon chromosome
Accession NZ_AP025340
Organism Bacillus paralicheniformis strain J36TS2

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag L6450_RS20620 Protein ID WP_003179128.1
Coordinates 4093455..4093805 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag L6450_RS20615 Protein ID WP_006638778.1
Coordinates 4093169..4093450 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6450_RS20595 4088279..4089763 + 1485 WP_041817155.1 PH domain-containing protein -
L6450_RS20600 4089760..4090359 - 600 WP_020450255.1 rhomboid family intramembrane serine protease -
L6450_RS20605 4090703..4091662 + 960 WP_165395380.1 outer membrane lipoprotein carrier protein LolA -
L6450_RS20610 4091888..4093057 + 1170 WP_041817156.1 alanine racemase -
L6450_RS20615 4093169..4093450 + 282 WP_006638778.1 hypothetical protein Antitoxin
L6450_RS20620 4093455..4093805 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6450_RS20625 4093923..4094750 + 828 WP_020450258.1 RsbT co-antagonist protein RsbRA -
L6450_RS20630 4094754..4095119 + 366 WP_020450259.1 STAS domain-containing protein -
L6450_RS20635 4095122..4095523 + 402 WP_020450260.1 anti-sigma regulatory factor -
L6450_RS20640 4095534..4096541 + 1008 WP_020450261.1 PP2C family protein-serine/threonine phosphatase -
L6450_RS20645 4096600..4096926 + 327 WP_020450262.1 anti-sigma factor antagonist -
L6450_RS20650 4096926..4097408 + 483 WP_020450263.1 anti-sigma B factor RsbW -
L6450_RS20655 4097374..4098165 + 792 WP_023857858.1 RNA polymerase sigma factor SigB -
L6450_RS20660 4098162..4098761 + 600 WP_020450265.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T40277 WP_003179128.1 NZ_AP025340:4093455-4093805 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T40277 NZ_AP025340:4093455-4093805 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT40277 WP_006638778.1 NZ_AP025340:4093169-4093450 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT40277 NZ_AP025340:4093169-4093450 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References