Detailed information of TA system TA040276

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 551214..552133
• Replicon: chromosome
• Accession: NZ_AP025340
• Organism: Bacillus paralicheniformis strain J36TS2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: L6450_RS02945
• Protein ID: WP_020451047.1
• Coordinates: 551387..552133 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: -
• Locus tag: L6450_RS02940
• Protein ID: WP_020451046.1
• Coordinates: 551214..551387 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6450_RS02915	546633..547730	+	1098	WP_020451042.1	mannonate dehydratase	-
L6450_RS02920	547706..548554	+	849	WP_020451043.1	SDR family oxidoreductase	-
L6450_RS02925	548582..549595	+	1014	WP_020451044.1	zinc-binding alcohol dehydrogenase family protein	-
L6450_RS02930	549647..550918	+	1272	WP_020451045.1	MFS transporter	-
L6450_RS02935	550989..551120	-	132	WP_023856733.1	hypothetical protein	-
L6450_RS02940	551214..551387	-	174	WP_020451046.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
L6450_RS02945	551387..552133	-	747	WP_020451047.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6450_RS02950	552243..553241	-	999	WP_020451048.1	inorganic phosphate transporter	-
L6450_RS02955	553254..553871	-	618	WP_020451049.1	DUF47 domain-containing protein	-
L6450_RS02960	554203..555960	+	1758	WP_020451051.1	gamma-glutamyltransferase	-
L6450_RS02965	556097..556741	+	645	WP_020451052.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29435.89 Da
• Isoelectric Point: 4.6065

>T40276 WP_020451047.1 NZ_AP025340:c552133-551387 [Bacillus paralicheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKKLTEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLERKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T40276 NZ_AP025340:c552133-551387 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTTTTGGCGCTGGCTCTGTATGTAGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACGTGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACTTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCCTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGGATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6926.00 Da
• Isoelectric Point: 10.9186

>AT40276 WP_020451046.1 NZ_AP025340:c551387-551214 [Bacillus paralicheniformis]
MEPAFQQDHESAKKRLAFKMFKRQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT40276 NZ_AP025340:c551387-551214 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA