Detailed information of TA system TA040275

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3772607..3773243
• Replicon: chromosome
• Accession: NZ_AP025339
• Organism: Bacillus paralicheniformis strain J25TS1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: M5P3Q9
• Locus tag: L6432_RS18810
• Protein ID: WP_003179128.1
• Coordinates: 3772893..3773243 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M5PDU2
• Locus tag: L6432_RS18805
• Protein ID: WP_006638778.1
• Coordinates: 3772607..3772888 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6432_RS18785	3767717..3769201	+	1485	WP_043054080.1	PH domain-containing protein	-
L6432_RS18790	3769198..3769797	-	600	WP_020450255.1	rhomboid family intramembrane serine protease	-
L6432_RS18795	3770141..3771100	+	960	WP_165395380.1	outer membrane lipoprotein carrier protein LolA	-
L6432_RS18800	3771326..3772495	+	1170	WP_023857864.1	alanine racemase	-
L6432_RS18805	3772607..3772888	+	282	WP_006638778.1	hypothetical protein	Antitoxin
L6432_RS18810	3772893..3773243	+	351	WP_003179128.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
L6432_RS18815	3773361..3774188	+	828	WP_023857863.1	RsbT co-antagonist protein RsbRA	-
L6432_RS18820	3774192..3774557	+	366	WP_031305225.1	STAS domain-containing protein	-
L6432_RS18825	3774560..3774961	+	402	WP_023857861.1	anti-sigma regulatory factor	-
L6432_RS18830	3774972..3775979	+	1008	WP_020450261.1	PP2C family protein-serine/threonine phosphatase	-
L6432_RS18835	3776038..3776364	+	327	WP_020450262.1	anti-sigma factor antagonist	-
L6432_RS18840	3776364..3776846	+	483	WP_020450263.1	anti-sigma B factor RsbW	-
L6432_RS18845	3776812..3777603	+	792	WP_023857858.1	RNA polymerase sigma factor SigB	-
L6432_RS18850	3777600..3778199	+	600	WP_023857857.1	PP2C family serine/threonine-protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12990.03 Da
• Isoelectric Point: 5.7234

>T40275 WP_003179128.1 NZ_AP025339:3772893-3773243 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Nucleotide

• Length: 351 bp

>T40275 NZ_AP025339:3772893-3773243 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10649.03 Da
• Isoelectric Point: 4.6920

>AT40275 WP_006638778.1 NZ_AP025339:3772607-3772888 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Nucleotide

• Length: 282 bp

>AT40275 NZ_AP025339:3772607-3772888 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A6I7FHI4

Antitoxin

• AlphaFold DB: M5PDU2