Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3772607..3773243 Replicon chromosome
Accession NZ_AP025339
Organism Bacillus paralicheniformis strain J25TS1

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag L6432_RS18810 Protein ID WP_003179128.1
Coordinates 3772893..3773243 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag L6432_RS18805 Protein ID WP_006638778.1
Coordinates 3772607..3772888 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6432_RS18785 3767717..3769201 + 1485 WP_043054080.1 PH domain-containing protein -
L6432_RS18790 3769198..3769797 - 600 WP_020450255.1 rhomboid family intramembrane serine protease -
L6432_RS18795 3770141..3771100 + 960 WP_165395380.1 outer membrane lipoprotein carrier protein LolA -
L6432_RS18800 3771326..3772495 + 1170 WP_023857864.1 alanine racemase -
L6432_RS18805 3772607..3772888 + 282 WP_006638778.1 hypothetical protein Antitoxin
L6432_RS18810 3772893..3773243 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
L6432_RS18815 3773361..3774188 + 828 WP_023857863.1 RsbT co-antagonist protein RsbRA -
L6432_RS18820 3774192..3774557 + 366 WP_031305225.1 STAS domain-containing protein -
L6432_RS18825 3774560..3774961 + 402 WP_023857861.1 anti-sigma regulatory factor -
L6432_RS18830 3774972..3775979 + 1008 WP_020450261.1 PP2C family protein-serine/threonine phosphatase -
L6432_RS18835 3776038..3776364 + 327 WP_020450262.1 anti-sigma factor antagonist -
L6432_RS18840 3776364..3776846 + 483 WP_020450263.1 anti-sigma B factor RsbW -
L6432_RS18845 3776812..3777603 + 792 WP_023857858.1 RNA polymerase sigma factor SigB -
L6432_RS18850 3777600..3778199 + 600 WP_023857857.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T40275 WP_003179128.1 NZ_AP025339:3772893-3773243 [Bacillus paralicheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T40275 NZ_AP025339:3772893-3773243 [Bacillus paralicheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGATTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT40275 WP_006638778.1 NZ_AP025339:3772607-3772888 [Bacillus paralicheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT40275 NZ_AP025339:3772607-3772888 [Bacillus paralicheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTGCGCGAGCGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATTAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References