Detailed information of TA system TA040274

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 282717..283636
• Replicon: chromosome
• Accession: NZ_AP025339
• Organism: Bacillus paralicheniformis strain J25TS1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: A0A6I7TYV7
• Locus tag: L6432_RS01500
• Protein ID: WP_023856735.1
• Coordinates: 282890..283636 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: A0A6I7TM13
• Locus tag: L6432_RS01495
• Protein ID: WP_023856734.1
• Coordinates: 282717..282890 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6432_RS01470	278142..279239	+	1098	WP_023856730.1	mannonate dehydratase	-
L6432_RS01475	279215..280063	+	849	WP_023856787.1	SDR family oxidoreductase	-
L6432_RS01480	280089..281102	+	1014	WP_023856731.1	zinc-binding alcohol dehydrogenase family protein	-
L6432_RS01485	281154..282425	+	1272	WP_023856732.1	MFS transporter	-
L6432_RS01490	282492..282623	-	132	WP_023856733.1	hypothetical protein	-
L6432_RS01495	282717..282890	-	174	WP_023856734.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
L6432_RS01500	282890..283636	-	747	WP_023856735.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
L6432_RS01505	283746..284744	-	999	WP_023856736.1	inorganic phosphate transporter	-
L6432_RS01510	284757..285374	-	618	WP_023856737.1	DUF47 domain-containing protein	-
L6432_RS01515	285707..287464	+	1758	WP_023856738.1	gamma-glutamyltransferase	-
L6432_RS01520	287599..288243	+	645	WP_023856740.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29421.87 Da
• Isoelectric Point: 4.6015

>T40274 WP_023856735.1 NZ_AP025339:c283636-282890 [Bacillus paralicheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKKLTEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLERKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEDGDD

Nucleotide

• Length: 747 bp

>T40274 NZ_AP025339:c283636-282890 [Bacillus paralicheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTCATTGTATTGGCGCTGGCTCTGTATGTGGCTGCCGTGTGGCGTTTTGA
AAAAAAGCTCACCGAAAAAACGGTCGCGATCCGAAAAACGTGGTACCTGCTTTATGTTATCGGCGCCGTGATTTATTGGA
CGCACGATCCGCAGTCGATTTTCACAAACCCGCTTCATTACTTGATCGTTGCCGTTTTTTTCACGCTGACAGACGCTTTT
ATTTTCTTAAACGCTTACTTTAAAAAGCTCGGCAGCTCCGAACTCGCGACAGACACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACATATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCATATGCAGAAGGGATCGAAAAGCTCATCAAACGATTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGCTTCGATGTTCAAGAAAAGCTTGA
GCGCAAAGACGTTTATTATGAAGAAAATGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGACTACCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGACGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6897.99 Da
• Isoelectric Point: 10.8356

>AT40274 WP_023856734.1 NZ_AP025339:c282890-282717 [Bacillus paralicheniformis]
MEPAFQQDHESAKKRLAFKMFKKQSKTNIAKYEVSPHTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT40274 NZ_AP025339:c282890-282717 [Bacillus paralicheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCATGAAAGCGCAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAAGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCCATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: A0A6I7TYV7

Antitoxin

• AlphaFold DB: A0A6I7TM13