Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 32165..32808 Replicon plasmid pTMTA97341
Accession NZ_AP025335
Organism Phytobacter diazotrophicus strain TA9734

Toxin (Protein)


Gene name vagD Uniprot ID A0A348DMH6
Locus tag L6445_RS27555 Protein ID WP_047368852.1
Coordinates 32165..32581 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A348DMH7
Locus tag L6445_RS27560 Protein ID WP_047368851.1
Coordinates 32578..32808 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6445_RS27545 (PDTA9734_54440) 28940..29182 + 243 WP_047368854.1 hypothetical protein -
L6445_RS27550 (PDTA9734_54450) 30749..31978 + 1230 WP_047368853.1 HDOD domain-containing protein -
L6445_RS27555 (PDTA9734_54460) 32165..32581 - 417 WP_047368852.1 type II toxin-antitoxin system VapC family toxin Toxin
L6445_RS27560 (PDTA9734_54470) 32578..32808 - 231 WP_047368851.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L6445_RS27565 (PDTA9734_54480) 33153..33413 + 261 WP_007374405.1 type II toxin-antitoxin system ParD family antitoxin -
L6445_RS27570 (PDTA9734_54490) 33400..33696 + 297 WP_047368850.1 type II toxin-antitoxin system RelE/ParE family toxin -
L6445_RS27575 (PDTA9734_54500) 33926..34360 - 435 WP_007374421.1 hypothetical protein -
L6445_RS27580 (PDTA9734_54510) 34578..35978 - 1401 WP_007374420.1 copper resistance membrane spanning protein PcoS -
L6445_RS27585 (PDTA9734_54520) 35975..36655 - 681 WP_001188930.1 copper response regulator transcription factor PcoR -
L6445_RS27590 (PDTA9734_54530) 36710..37639 - 930 WP_000998778.1 copper resistance inner membrane protein PcoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..174856 174856


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15042.32 Da        Isoelectric Point: 7.2449

>T40271 WP_047368852.1 NZ_AP025335:c32581-32165 [Phytobacter diazotrophicus]
VNKTYMLDTNICSLIMREQPEAVLKRLEQAVLRNHRTVISAITYQEMRSGATGPKASPRHIALVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAVAAGAILVTNNTREFERVPGLVLEDWVN

Download         Length: 417 bp

>T40271 NZ_AP025335:c32581-32165 [Phytobacter diazotrophicus]
GTGAACAAAACGTATATGCTGGACACTAACATCTGCTCATTAATCATGCGCGAGCAACCGGAAGCGGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCAACCACCGCACTGTGATCTCGGCCATCACCTACCAGGAGATGCGCTCCGGCGCCACGGGCC
CGAAGGCTTCGCCGCGCCATATTGCGCTGGTTGATGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCG
GCGGTCGACGCTACCACGGACATCCGGGTGGCGCTGCGCCTCGCAGGCACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCATGCGGTTGCCGCCGGCGCCATCCTGGTGACCAACAATACAAGAGAGTTTGAACGGGTTCCGGGTCTGGTGCTGG
AAGACTGGGTAAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8780.97 Da        Isoelectric Point: 4.6583

>AT40271 WP_047368851.1 NZ_AP025335:c32808-32578 [Phytobacter diazotrophicus]
MRTVSIFKNGNNRAIRLPRDMDFEGVSELEIVREGDRIILRPVRPTWGSFAKMEKADPDFMAEREDVVSDEGRVDL

Download         Length: 231 bp

>AT40271 NZ_AP025335:c32808-32578 [Phytobacter diazotrophicus]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGTGACATGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGAGACCGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGAAGATGG
AAAAAGCAGACCCGGACTTTATGGCGGAGCGTGAGGACGTTGTCAGTGACGAAGGACGGGTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DMH6

Antitoxin

Source ID Structure
AlphaFold DB A0A348DMH7

References