Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4364083..4364702 Replicon chromosome
Accession NZ_AP025334
Organism Phytobacter diazotrophicus strain TA9734

Toxin (Protein)


Gene name Hha Uniprot ID A0A348DII6
Locus tag L6445_RS21055 Protein ID WP_041852465.1
Coordinates 4364484..4364702 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A348DII5
Locus tag L6445_RS21050 Protein ID WP_041852464.1
Coordinates 4364083..4364457 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6445_RS21040 (4359194) 4359194..4360390 + 1197 WP_041852462.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
L6445_RS21045 (4360413) 4360413..4363562 + 3150 WP_125125202.1 multidrug efflux RND transporter permease subunit AcrB -
L6445_RS21050 (4364083) 4364083..4364457 + 375 WP_041852464.1 Hha toxicity modulator TomB Antitoxin
L6445_RS21055 (4364484) 4364484..4364702 + 219 WP_041852465.1 HHA domain-containing protein Toxin
L6445_RS21060 (4364771) 4364771..4365337 + 567 WP_125125203.1 maltose O-acetyltransferase -
L6445_RS21065 (4365437) 4365437..4365904 + 468 WP_041852467.1 YlaC family protein -
L6445_RS21070 (4365882) 4365882..4367327 - 1446 WP_125125204.1 PLP-dependent aminotransferase family protein -
L6445_RS21075 (4367427) 4367427..4368128 + 702 WP_041852469.1 GNAT family protein -
L6445_RS21080 (4368125) 4368125..4368265 - 141 WP_006818844.1 type B 50S ribosomal protein L36 -
L6445_RS21085 (4368269) 4368269..4368529 - 261 WP_041852470.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8685.14 Da        Isoelectric Point: 8.9039

>T40269 WP_041852465.1 NZ_AP025334:4364484-4364702 [Phytobacter diazotrophicus]
MSDKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELPDHELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T40269 NZ_AP025334:4364484-4364702 [Phytobacter diazotrophicus]
ATGTCTGATAAGCCATTAACCAAAATTGATTATTTGATGCGATTACGACGTTGTCAGACAATTGACACGCTCGAACGCGT
TATTGAAAAAAATAAATACGAACTGCCTGACCATGAACTGACGGTGTTTTATTCAGCAGCCGATCATCGTCTGGCTGAGC
TAACCATGAATAAACTGTATGACAAAATCCCTCCTTCTGTGTGGAAATTCGTACGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14600.44 Da        Isoelectric Point: 5.5072

>AT40269 WP_041852464.1 NZ_AP025334:4364083-4364457 [Phytobacter diazotrophicus]
MDEYSPKRHDIAQLKFLCETLYHDCLLNLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSKANPVSHSC

Download         Length: 375 bp

>AT40269 NZ_AP025334:4364083-4364457 [Phytobacter diazotrophicus]
ATGGACGAATACTCGCCAAAAAGGCATGATATCGCGCAGCTTAAATTTCTCTGCGAAACGTTGTACCATGATTGTCTCCT
GAATCTTGAAGAAAGCCATCATGGATGGGTTAACGATCCTACTTCGGCAATCAATTTGCAGTTAAATGAGTTGATTGAAC
ATATAGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAAGATAATAAACTGATTGAGCAGATAGACGAATACCTT
GATGATACGTTTATGCTGTTCAGTAGCTACGGTATCAACACACAAGACTTGCAGAAATGGCGTAAATCGGGAAACCGATT
GTTCCGCTGTTTCGTCAATGTGAGCAAGGCTAACCCTGTTAGTCACTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DII6

Antitoxin

Source ID Structure
AlphaFold DB A0A348DII5

References