Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 2786970..2787613 Replicon chromosome
Accession NZ_AP025334
Organism Phytobacter diazotrophicus strain TA9734

Toxin (Protein)


Gene name vagD Uniprot ID A0A348DE83
Locus tag L6445_RS13400 Protein ID WP_071194208.1
Coordinates 2787197..2787613 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A348DE82
Locus tag L6445_RS13395 Protein ID WP_071194209.1
Coordinates 2786970..2787200 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6445_RS13375 (2783132) 2783132..2783509 + 378 WP_108701294.1 VOC family protein -
L6445_RS13380 (2783580) 2783580..2784533 + 954 WP_039079008.1 2-hydroxyacid dehydrogenase -
L6445_RS13385 (2784585) 2784585..2785349 + 765 WP_039079009.1 SDR family oxidoreductase -
L6445_RS13390 (2785426) 2785426..2786712 - 1287 WP_125124672.1 MFS transporter -
L6445_RS13395 (2786970) 2786970..2787200 + 231 WP_071194209.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
L6445_RS13400 (2787197) 2787197..2787613 + 417 WP_071194208.1 PIN domain-containing protein Toxin
L6445_RS13405 (2787876) 2787876..2788283 - 408 WP_125125677.1 nuclear transport factor 2 family protein -
L6445_RS13410 (2788568) 2788568..2789566 + 999 WP_039079015.1 ABC transporter substrate-binding protein -
L6445_RS13415 (2789566) 2789566..2790603 + 1038 WP_125124673.1 iron chelate uptake ABC transporter family permease subunit -
L6445_RS13420 (2790603) 2790603..2791364 + 762 WP_071194204.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15050.57 Da        Isoelectric Point: 9.2345

>T40259 WP_071194208.1 NZ_AP025334:2787197-2787613 [Phytobacter diazotrophicus]
MKKTFMLDTNICSFIMREHPESVIKRLEQAVLRNNRIVVSAITYAEMRFGAIGKKASPHHAKLVDAFCARLDAVLAWDRA
AVDATTQIKLALALAGTPIGPNDTAIAGHAIAAGVVLVTNNTREFERVPGLILEDWAK

Download         Length: 417 bp

>T40259 NZ_AP025334:2787197-2787613 [Phytobacter diazotrophicus]
ATGAAGAAAACCTTTATGCTCGACACTAATATTTGCTCATTCATCATGCGCGAGCATCCGGAAAGCGTTATTAAGCGTCT
GGAGCAGGCAGTGTTGCGCAACAATCGAATCGTAGTATCGGCTATCACCTATGCGGAAATGCGCTTCGGCGCGATCGGTA
AAAAGGCGTCGCCGCATCATGCGAAGCTGGTTGATGCGTTCTGTGCTCGCCTTGATGCCGTGCTCGCCTGGGACCGGGCG
GCAGTGGATGCCACTACGCAAATAAAGCTTGCGCTGGCGTTGGCTGGTACGCCGATTGGTCCGAACGATACCGCGATTGC
CGGGCATGCTATTGCCGCTGGCGTCGTGCTGGTAACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTGATTCTGG
AAGACTGGGCAAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8783.91 Da        Isoelectric Point: 4.3654

>AT40259 WP_071194209.1 NZ_AP025334:2786970-2787200 [Phytobacter diazotrophicus]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIIREGDCIILRPVRPTWSSFAQLEKADPDFMAEREDVVSDEGRFDL

Download         Length: 231 bp

>AT40259 NZ_AP025334:2786970-2787200 [Phytobacter diazotrophicus]
ATGCGAACCGTATCCATCTTCAAAAACGGCAACAATCGTGCCATCCGTCTGCCTCGCGATCTGGATTTCGAAGGAGTGAG
CGAACTGGAGATCATCAGGGAAGGGGACTGCATCATCCTGCGCCCGGTGCGACCGACGTGGAGTTCTTTCGCGCAATTAG
AAAAAGCCGACCCGGATTTTATGGCAGAACGTGAAGATGTGGTCAGTGATGAGGGACGCTTTGATCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DE83

Antitoxin

Source ID Structure
AlphaFold DB A0A348DE82

References