Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1953395..1953985 Replicon chromosome
Accession NZ_AP025334
Organism Phytobacter diazotrophicus strain TA9734

Toxin (Protein)


Gene name pemK Uniprot ID -
Locus tag L6445_RS09130 Protein ID WP_125124239.1
Coordinates 1953653..1953985 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID -
Locus tag L6445_RS09125 Protein ID WP_125124238.1
Coordinates 1953395..1953652 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6445_RS09100 (1949002) 1949002..1949337 - 336 WP_041851877.1 gamma-glutamylcyclotransferase family protein -
L6445_RS09105 (1949464) 1949464..1949754 - 291 WP_079496374.1 DUF3601 domain-containing protein -
L6445_RS09110 (1950012) 1950012..1950551 - 540 WP_079496546.1 ester cyclase -
L6445_RS09115 (1950850) 1950850..1951959 - 1110 WP_125124236.1 alkene reductase -
L6445_RS09120 (1952060) 1952060..1952971 + 912 WP_125124237.1 LysR family transcriptional regulator -
L6445_RS09125 (1953395) 1953395..1953652 + 258 WP_125124238.1 antitoxin Antitoxin
L6445_RS09130 (1953653) 1953653..1953985 + 333 WP_125124239.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
L6445_RS09140 (1954386) 1954386..1955819 + 1434 WP_103245488.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
L6445_RS09150 (1956104) 1956104..1956331 - 228 WP_125124240.1 hypothetical protein -
L6445_RS09155 (1956548) 1956548..1958008 + 1461 WP_125124241.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Antitoxin PemI-like

(5-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11781.49 Da        Isoelectric Point: 6.9812

>T40257 WP_125124239.1 NZ_AP025334:1953653-1953985 [Phytobacter diazotrophicus]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSPASFNALTRLPVVVPVTSGGNFARAAGFAVSLEGAGTKTTGVIRCDQPRTI
DMDARNDKRLERIPDAVVNEVLARLEAILS

Download         Length: 333 bp

>T40257 NZ_AP025334:1953653-1953985 [Phytobacter diazotrophicus]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCGACCACCGGGCATGAACAAAGCGGAAAACGTCCAGTGCTTAT
CGTTTCTCCCGCGTCATTTAATGCACTCACCCGTTTGCCCGTTGTTGTTCCGGTCACCAGTGGCGGAAACTTTGCCCGAG
CGGCGGGTTTTGCCGTCTCACTGGAAGGTGCGGGAACGAAAACCACGGGTGTGATTCGCTGCGATCAGCCCAGAACCATT
GATATGGACGCCCGAAACGACAAGCGTCTGGAACGTATACCCGATGCTGTCGTGAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGTTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9272.74 Da        Isoelectric Point: 4.5799

>AT40257 WP_125124238.1 NZ_AP025334:1953395-1953652 [Phytobacter diazotrophicus]
MYIARLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIENGCLIIEPQKRPHYSLEELLAQCDPHAEISEEDRQWIDGPAA
GKELL

Download         Length: 258 bp

>AT40257 NZ_AP025334:1953395-1953652 [Phytobacter diazotrophicus]
ATGTATATCGCTCGCCTTAAAAAGGTTGGAGGATCCGTCATGCTGGCGGTTCCTCCTGCCGTATTGAAAACCCTGGGGCT
GTCGACGGATAGCGAAGTGGGTATGACCATTGAGAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCACATTACTCGC
TTGAGGAATTGCTGGCGCAGTGTGATCCGCACGCCGAAATCAGCGAAGAGGATCGGCAATGGATTGATGGGCCTGCGGCG
GGCAAGGAGCTTCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References