Detailed information of TA system TA040252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 1184345..1185588
• Replicon: chromosome
• Accession: NZ_AP025333
• Organism: Streptococcus ruminantium strain GUT-189

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: G7SEB4
• Locus tag: L6411_RS05785
• Protein ID: WP_001235242.1
• Coordinates: 1184345..1185112 (-)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: L6411_RS05790
• Protein ID: WP_000578719.1
• Coordinates: 1185112..1185588 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6411_RS05770 (GUT189_11020)	1180733..1181098	-	366	WP_000431640.1	MobC family plasmid mobilization relaxosome protein	-
L6411_RS05775 (GUT189_11030)	1181101..1181463	-	363	WP_000140674.1	SAG1252 family conjugative relaxosome accessory protein	-
L6411_RS05780 (GUT189_11040)	1181801..1184344	-	2544	WP_000601012.1	AAA family ATPase	-
L6411_RS05785 (GUT189_11050)	1184345..1185112	-	768	WP_001235242.1	type II toxin-antitoxin system toxin PezT	Toxin
L6411_RS05790 (GUT189_11060)	1185112..1185588	-	477	WP_000578719.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
L6411_RS05795 (GUT189_11070)	1185658..1185948	-	291	WP_000667951.1	hypothetical protein	-
L6411_RS05800 (GUT189_11080)	1185999..1186388	-	390	WP_000048066.1	DUF5945 family protein	-
L6411_RS05805 (GUT189_11090)	1186385..1186612	-	228	WP_000110922.1	DUF5965 family protein	-
L6411_RS05810 (GUT189_11100)	1186640..1187740	-	1101	WP_000196859.1	toprim domain-containing protein	-
L6411_RS05815 (GUT189_11110)	1187804..1189495	-	1692	WP_050547274.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / erm(B) / tet(O)	-	1168821..1231471	62650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 28985.80 Da
• Isoelectric Point: 5.1126

>T40252 WP_001235242.1 NZ_AP025333:c1185112-1184345 [Streptococcus ruminantium]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEGSAAEVLQECLFGKWSKVEEEMV
KESELLLKELLEKNK

Nucleotide

• Length: 768 bp

>T40252 NZ_AP025333:c1185112-1184345 [Streptococcus ruminantium]
ATGAGACTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATCTTGCTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AGGAATTTCAAGGTAATATCATTATTATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAGGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAAGGCTACCATTTGCTGATTGAGGGAACCTTGCGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCACGATGGGATTGTTGAGAACTTGGTTGATAA
CTTGAGGGAGTTAGAAAGTGAAAATCTCTTTGACCAGATTCAAATTTATCAAAGAGATAGAACTTGTATCTATGATTCTG
AAACTGCTGAAGGTTCCGCAGCAGAAGTGCTACAAGAATGCCTATTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAAGGAATTACTTGAGAAAAATAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18266.77 Da
• Isoelectric Point: 4.6424

>AT40252 WP_000578719.1 NZ_AP025333:c1185588-1185112 [Streptococcus ruminantium]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSKLYRYQDSQDIVFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT40252 NZ_AP025333:c1185588-1185112 [Streptococcus ruminantium]
ATGATCGGAGACAATATAAAATCACTACGCCGAACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGTACCAGCACTGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTCGAAGATTACCATCTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCAAAGCTTTATAGATACCAGGATAGTCAAGATATTGTTTTTGATGATGA
ATCTAATCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATCAATACGAAAATTTACCTTGTCGCTACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGATGGTATAGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: G7SEB4