Detailed information of TA system TA040251

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 691605..692087
• Replicon: chromosome
• Accession: NZ_AP025333
• Organism: Streptococcus ruminantium strain GUT-189

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L6411_RS03375
• Protein ID: WP_237374390.1
• Coordinates: 691827..692087 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L6411_RS03370
• Protein ID: WP_024532383.1
• Coordinates: 691605..691826 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6411_RS03360 (GUT189_06160)	688577..690655	-	2079	WP_237374389.1	GAG-binding domain-containing protein	-
L6411_RS03365 (GUT189_06170)	691061..691405	+	345	WP_227985764.1	minor capsid protein	-
L6411_RS03370 (GUT189_06180)	691605..691826	+	222	WP_024532383.1	DUF6290 family protein	Antitoxin
L6411_RS03375 (GUT189_06190)	691827..692087	+	261	WP_237374390.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6411_RS03380 (GUT189_06200)	692201..692491	+	291	WP_237374391.1	hypothetical protein	-
L6411_RS03385 (GUT189_06210)	692693..693655	-	963	WP_155970832.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
L6411_RS03390 (GUT189_06220)	693865..696024	-	2160	WP_237374392.1	class 1b ribonucleoside-diphosphate reductase subunit alpha	-
L6411_RS03395 (GUT189_06230)	696067..696285	-	219	WP_024532387.1	glutaredoxin-like protein NrdH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	691076..715154	24078

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-86)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10290.82 Da
• Isoelectric Point: 9.6252

>T40251 WP_237374390.1 NZ_AP025333:691827-692087 [Streptococcus ruminantium]
MYHVEYSKKAQKQIRKLDKSIQRLLFAWVDKYLDGTDTPRTNGKGLTGNHANEWRYRIGDYRLICEIQEDKMVILALEFG
HRKDIY

Nucleotide

• Length: 261 bp

>T40251 NZ_AP025333:691827-692087 [Streptococcus ruminantium]
ATGTATCATGTAGAGTATTCAAAAAAGGCTCAAAAGCAGATCAGAAAGCTGGATAAATCTATCCAAAGGCTACTTTTTGC
TTGGGTAGATAAGTATTTAGACGGTACAGACACCCCACGAACTAACGGCAAGGGATTGACAGGAAACCACGCCAATGAAT
GGCGTTATCGTATCGGTGATTATAGACTGATATGCGAAATTCAAGAGGATAAGATGGTTATTCTAGCTCTTGAGTTTGGG
CATAGGAAAGATATTTACTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8515.55 Da
• Isoelectric Point: 4.6128

>AT40251 WP_024532383.1 NZ_AP025333:691605-691826 [Streptococcus ruminantium]
MSTVTIRLNQEEETFFKSYAQLTGQSLSSLFKKALERDIEDEYDLKIYKQAYAEYKADPETISHADFKKELGL

Nucleotide

• Length: 222 bp

>AT40251 NZ_AP025333:691605-691826 [Streptococcus ruminantium]
ATGAGTACAGTAACTATCAGATTAAATCAAGAAGAAGAAACATTTTTCAAAAGCTATGCTCAATTAACAGGGCAAAGTCT
TTCAAGTCTTTTCAAGAAAGCTCTTGAGCGTGATATTGAAGATGAGTATGATTTGAAAATTTATAAACAAGCCTATGCTG
AATACAAAGCAGACCCAGAGACAATTAGCCATGCTGATTTTAAGAAAGAGCTGGGCTTGTAA