Detailed information of TA system TA040248

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-couple_hipB
• Location: 1250461..1251704
• Replicon: chromosome
• Accession: NZ_AP025331
• Organism: Streptococcus ruminantium strain GUT-183

Toxin (Protein)

• Gene name: sezT
• Uniprot ID: G7SEB4
• Locus tag: SUT_RS06000
• Protein ID: WP_001235242.1
• Coordinates: 1250461..1251228 (-)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: sezA
• Uniprot ID: -
• Locus tag: SUT_RS06005
• Protein ID: WP_000578719.1
• Coordinates: 1251228..1251704 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SUT_RS05985 (GUT183_11500)	1246849..1247214	-	366	WP_000431640.1	MobC family plasmid mobilization relaxosome protein	-
SUT_RS05990 (GUT183_11510)	1247217..1247579	-	363	WP_000140674.1	SAG1252 family conjugative relaxosome accessory protein	-
SUT_RS05995 (GUT183_11520)	1247917..1250460	-	2544	WP_000601012.1	AAA family ATPase	-
SUT_RS06000 (GUT183_11530)	1250461..1251228	-	768	WP_001235242.1	type II toxin-antitoxin system toxin PezT	Toxin
SUT_RS06005 (GUT183_11540)	1251228..1251704	-	477	WP_000578719.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
SUT_RS06010 (GUT183_11550)	1251774..1252064	-	291	WP_000667951.1	hypothetical protein	-
SUT_RS06015 (GUT183_11560)	1252115..1252504	-	390	WP_000048066.1	DUF5945 family protein	-
SUT_RS06020 (GUT183_11570)	1252501..1252728	-	228	WP_000110922.1	DUF5965 family protein	-
SUT_RS06025 (GUT183_11580)	1252756..1253856	-	1101	WP_000196859.1	toprim domain-containing protein	-
SUT_RS06030 (GUT183_11590)	1253920..1255611	-	1692	WP_237373697.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	ant(6)-Ia / erm(B)	-	1207253..1266845	59592

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 28985.80 Da
• Isoelectric Point: 5.1126

>T40248 WP_001235242.1 NZ_AP025331:c1251228-1250461 [Streptococcus ruminantium]
MRLEEFSEAEFQKALQRTIRALTRGKTIPDQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEQLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLSSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPTQARATPKEHHDGIVENLVDNLRELESENLFDQIQIYQRDRTCIYDSETAEGSAAEVLQECLFGKWSKVEEEMV
KESELLLKELLEKNK

Nucleotide

• Length: 768 bp

>T40248 NZ_AP025331:c1251228-1250461 [Streptococcus ruminantium]
ATGAGACTGGAAGAATTTAGTGAGGCTGAGTTTCAGAAGGCCTTGCAGCGAACCATTCGGGCCTTAACCCGAGGAAAGAC
GATTCCAGATCAACCGAAAGCTATCTTGCTTGGTGGACAAAGCGGAGCAGGTAAGACGACTATTCATCGTATCAAGCAAA
AGGAATTTCAAGGTAATATCATTATTATCGATGGTGACAGCTACCGTTCTCAGCATCCCAATTACTTAGCCCTGCAAGAA
AAGTATGGCAAGGATAGCGTGGACTATACCAAGGGATTTGCAGGAAAAATGGTAGAACAGTTGGTTGATGAACTCAGTAC
GCAAGGCTACCATTTGCTGATTGAGGGAACCTTGCGAACTACTCAAGTTCCTCGTCAGACTGCTCAATTATTATCTTCTA
AAGGTTACCAAGTTTCCTTAGCAGTGATTGGTACCAAACCAGAGCTTTCTTATCTCAGCACCTTGATTCGTTACGAAGAG
CTTTATGCCATCGATCCGACTCAGGCTAGAGCAACACCAAAAGAACACCACGATGGGATTGTTGAGAACTTGGTTGATAA
CTTGAGGGAGTTAGAAAGTGAAAATCTCTTTGACCAGATTCAAATTTATCAAAGAGATAGAACTTGTATCTATGATTCTG
AAACTGCTGAAGGTTCCGCAGCAGAAGTGCTACAAGAATGCCTATTTGGAAAATGGAGCAAGGTAGAGGAGGAGATGGTG
AAGGAGAGTGAACTCCTACTTAAGGAATTACTTGAGAAAAATAAATAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18266.77 Da
• Isoelectric Point: 4.6424

>AT40248 WP_000578719.1 NZ_AP025331:c1251704-1251228 [Streptococcus ruminantium]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLTPVEDYHLTLKVE
VIKERGAAILSKLYRYQDSQDIVFDDESNPWILMSDDLAELINTKIYLVATFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT40248 NZ_AP025331:c1251704-1251228 [Streptococcus ruminantium]
ATGATCGGAGACAATATAAAATCACTACGCCGAACACACGATTTAACACAGCCAGAATTTGCGAAAATGGTTGGGATTTC
ACGCAATAGCCTGAGTCGCTACGAAAATGGTACCAGCACTGTTTCAACTGAACTCATCGACCGTATTTGTCAGAAATTTA
ACGTTTCTTATGTCGATATTGTAGGAGAGGACAAGATGTTAACACCTGTCGAAGATTACCATCTGACTTTAAAAGTAGAA
GTGATAAAAGAACGTGGAGCAGCCATTTTATCAAAGCTTTATAGATACCAGGATAGTCAAGATATTGTTTTTGATGATGA
ATCTAATCCTTGGATTTTGATGAGTGATGACTTGGCCGAATTGATCAATACGAAAATTTACCTTGTCGCTACTTTCGATG
AAATAGAGCGTTACAATGGCTATTTAGATGGTATAGAGCGGATGTTAGACATGGTGCATCATCGGGTGGTGGCTTAA

Structures

Toxin

• AlphaFold DB: G7SEB4