Detailed information of TA system TA040235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yoeB-relB/YoeB-YefM
• Location: 2437483..2437985
• Replicon: chromosome
• Accession: NZ_AP025280
• Organism: Aeromonas caviae strain NUITM-VA2

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: Q5XQD8
• Locus tag: L6426_RS11445
• Protein ID: WP_032492620.1
• Coordinates: 2437483..2437737 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0BEH6
• Locus tag: L6426_RS11450
• Protein ID: WP_032492621.1
• Coordinates: 2437734..2437985 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6426_RS11425 (NUITMVA2_21980)	2433862..2434422	-	561	WP_001161490.1	recombinase family protein	-
L6426_RS11430 (NUITMVA2_21990)	2434759..2435772	-	1014	WP_000845048.1	class 1 integron integrase IntI1	-
L6426_RS11435	2436019..2436807	+	789	Protein_2205	AadA family aminoglycoside 3''-O-nucleotidyltransferase	-
L6426_RS11440	2436856..2437410	+	555	WP_012695458.1	AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase	-
L6426_RS11445 (NUITMVA2_22010)	2437483..2437737	-	255	WP_032492620.1	Txe/YoeB family addiction module toxin	Toxin
L6426_RS11450 (NUITMVA2_22020)	2437734..2437985	-	252	WP_032492621.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
L6426_RS11455 (NUITMVA2_22030)	2438169..2438801	+	633	WP_000186237.1	type B-3 chloramphenicol O-acetyltransferase CatB3	-
L6426_RS11460 (NUITMVA2_22040)	2438958..2439305	+	348	WP_000679427.1	quaternary ammonium compound efflux SMR transporter QacE delta 1	-
L6426_RS11465 (NUITMVA2_22050)	2439299..2440138	+	840	WP_000259031.1	sulfonamide-resistant dihydropteroate synthase Sul1	-
L6426_RS11470 (NUITMVA2_22060)	2440543..2442084	+	1542	WP_000050481.1	IS91-like element ISCR1 family transposase	-
L6426_RS11475 (NUITMVA2_22070)	2442360..2442641	+	282	WP_015059004.1	sodium/hydrogen exchanger	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	ant(3'')-Ii-aac(6')-IId / catB3 / sul1 / rmtB / blaVIM-4 / mph(A)	adeG	2430892..2467953	37061
	-	inside	Integron	-	-	2434759..2438855	4096

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10272.81 Da
• Isoelectric Point: 9.3585

>T40235 WP_032492620.1 NZ_AP025280:c2437737-2437483 [Aeromonas caviae]
MRLIFSDDAWEDYLFWQKQDRRMVDRINKLIKETTREPFSGVGKPEPLKHALAGYWSRRITDEHRMVYKVADDALWIVQL
KYHY

Nucleotide

• Length: 255 bp

>T40235 NZ_AP025280:c2437737-2437483 [Aeromonas caviae]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9244.40 Da
• Isoelectric Point: 4.7769

>AT40235 WP_032492621.1 NZ_AP025280:c2437985-2437734 [Aeromonas caviae]
MDAITYTHARANLAGTMDRVCNDHEPVIITRNGDQSVVILSLEDFQALEETAYLLRSPANAKRLFKSIEQLEAGRGQARE
LAE

Nucleotide

• Length: 252 bp

>AT40235 NZ_AP025280:c2437985-2437734 [Aeromonas caviae]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Structures

Toxin

• AlphaFold DB: A0A1V0BEH9

Antitoxin

• AlphaFold DB: A0A1V0BEH6