Detailed information of TA system TA040232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 153562..154109
• Replicon: plasmid pMRCP2
• Accession: NZ_AP025274
• Organism: Pseudomonas alcaligenes strain MRCP2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: L6431_RS22920
• Protein ID: WP_116803468.1
• Coordinates: 153562..153837 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: L6431_RS22925
• Protein ID: WP_061194820.1
• Coordinates: 153837..154109 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
L6431_RS22885 (MRCP2_p2080)	148997..150079	-	1083	WP_237046870.1	hypothetical protein	-
L6431_RS22890 (MRCP2_p2090)	150302..150553	-	252	WP_237046871.1	hypothetical protein	-
L6431_RS22895 (MRCP2_p2100)	150632..151633	-	1002	WP_237046872.1	hypothetical protein	-
L6431_RS22900 (MRCP2_p2110)	151778..152335	-	558	WP_237046873.1	hypothetical protein	-
L6431_RS22905 (MRCP2_p2120)	152401..152898	-	498	WP_132706187.1	hypothetical protein	-
L6431_RS22910 (MRCP2_p2130)	152895..153095	-	201	WP_088922269.1	hypothetical protein	-
L6431_RS22915 (MRCP2_p2140)	153296..153559	-	264	WP_171953601.1	hypothetical protein	-
L6431_RS22920 (MRCP2_p2150)	153562..153837	-	276	WP_116803468.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
L6431_RS22925 (MRCP2_p2160)	153837..154109	-	273	WP_061194820.1	ribbon-helix-helix domain-containing protein	Antitoxin
L6431_RS22930 (MRCP2_p2170)	154115..154678	-	564	WP_116803469.1	hypothetical protein	-
L6431_RS22935 (MRCP2_p2180)	154675..155124	-	450	WP_061199210.1	hypothetical protein	-
L6431_RS22940 (MRCP2_p2190)	155140..155409	-	270	WP_061194823.1	hypothetical protein	-
L6431_RS22945 (MRCP2_p2200)	155429..156199	-	771	WP_061198833.1	DUF2807 domain-containing protein	-
L6431_RS22950	156334..156399	+	66	Protein_200	hypothetical protein	-
L6431_RS22955 (MRCP2_p2210)	156474..158153	+	1680	WP_000179844.1	DDE-type integrase/transposase/recombinase	-
L6431_RS22960 (MRCP2_p2220)	158156..159064	+	909	WP_003155746.1	TniB family NTP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaIMP-1 / fosE	-	1..323125	323125

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (10-47)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10673.22 Da
• Isoelectric Point: 7.2022

>T40232 WP_116803468.1 NZ_AP025274:c153837-153562 [Pseudomonas alcaligenes]
MELKWTSKALSDLARLYEFLAAVNQPAAARTVQQLTAAPTTLLTNPRIGERLEEFDPRDVRRILVGHYEMRYEIEGSTIY
LLRLWHTREDR

Nucleotide

• Length: 276 bp

>T40232 NZ_AP025274:c153837-153562 [Pseudomonas alcaligenes]
ATGGAGTTGAAGTGGACGAGCAAGGCCCTATCCGATCTGGCCCGCCTCTATGAGTTCCTTGCAGCGGTGAACCAGCCCGC
TGCGGCGCGCACAGTGCAGCAGCTCACCGCAGCGCCGACGACGCTGCTGACCAACCCGCGCATCGGCGAGCGGCTGGAAG
AGTTCGATCCGCGCGACGTGCGACGGATTCTGGTCGGGCACTACGAGATGCGCTACGAAATCGAAGGCTCCACCATCTAC
CTGCTGCGCCTGTGGCACACCCGCGAGGATCGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10054.25 Da
• Isoelectric Point: 4.8616

>AT40232 WP_061194820.1 NZ_AP025274:c154109-153837 [Pseudomonas alcaligenes]
MAATAKTRSVTAHVPVELAERVDEIAERLERSKNWIVKQALSAWIDQEEERSRLTREALADVDAGRVIDHQAVQAWADSL
STDTPLPVPH

Nucleotide

• Length: 273 bp

>AT40232 NZ_AP025274:c154109-153837 [Pseudomonas alcaligenes]
ATGGCCGCAACAGCTAAAACCCGATCCGTCACCGCCCACGTCCCTGTAGAGCTGGCCGAACGTGTTGATGAGATTGCCGA
GCGCCTGGAGCGCTCCAAGAACTGGATCGTCAAGCAAGCCCTGTCCGCCTGGATCGACCAGGAAGAAGAGCGCAGCCGCC
TGACCCGCGAGGCTCTGGCCGATGTGGATGCCGGCCGCGTGATCGACCATCAGGCCGTCCAGGCCTGGGCCGACAGCCTG
AGCACAGACACCCCGCTGCCGGTACCGCACTGA