Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 1884790..1885483 Replicon chromosome
Accession NZ_AP025273
Organism Pseudomonas alcaligenes strain MRCP2

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag L6431_RS08920 Protein ID WP_003151133.1
Coordinates 1884790..1885167 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag L6431_RS08925 Protein ID WP_001172026.1
Coordinates 1885148..1885483 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6431_RS08905 1879901..1880746 - 846 WP_003464995.1 AraC family transcriptional regulator -
L6431_RS08910 1880983..1884012 - 3030 WP_024015041.1 Tn3 family transposase -
L6431_RS08915 1883996..1884598 - 603 WP_010465829.1 recombinase family protein -
L6431_RS08920 1884790..1885167 + 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
L6431_RS08925 1885148..1885483 + 336 WP_001172026.1 helix-turn-helix transcriptional regulator Antitoxin
L6431_RS08930 1885498..1885803 + 306 WP_223198361.1 transposase -
L6431_RS08935 1885856..1886182 + 327 WP_000091614.1 hypothetical protein -
L6431_RS08940 1886179..1886559 + 381 WP_001054412.1 hypothetical protein -
L6431_RS08945 1886716..1887018 + 303 WP_237046571.1 DUF3391 domain-containing protein -
L6431_RS08950 1887110..1887979 + 870 WP_223198360.1 HD-GYP domain-containing protein -
L6431_RS08955 1888389..1888628 + 240 WP_034011918.1 ribbon-helix-helix domain-containing protein -
L6431_RS08960 1888628..1889038 + 411 WP_034011919.1 putative toxin-antitoxin system toxin component, PIN family -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T40229 WP_003151133.1 NZ_AP025273:1884790-1885167 [Pseudomonas alcaligenes]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T40229 NZ_AP025273:1884790-1885167 [Pseudomonas alcaligenes]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGTGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGGAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTACGAAATGCAAAAACGAATCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT40229 WP_001172026.1 NZ_AP025273:1885148-1885483 [Pseudomonas alcaligenes]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT40229 NZ_AP025273:1885148-1885483 [Pseudomonas alcaligenes]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References