Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | Txe-Axe/Txe-RelB |
| Location | 31160..31679 | Replicon | plasmid pEK4S |
| Accession | NZ_AP025272 | ||
| Organism | Enterococcus faecalis strain K-4 | ||
Toxin (Protein)
| Gene name | Txe | Uniprot ID | S4D6W6 |
| Locus tag | EFK4_RS13850 | Protein ID | WP_016617334.1 |
| Coordinates | 31422..31679 (+) | Length | 86 a.a. |
Antitoxin (Protein)
| Gene name | Axe | Uniprot ID | - |
| Locus tag | EFK4_RS13845 | Protein ID | WP_101732003.1 |
| Coordinates | 31160..31429 (+) | Length | 90 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EFK4_RS13810 (EFK4_27120) | 26400..26771 | - | 372 | WP_236919507.1 | hypothetical protein | - |
| EFK4_RS13815 (EFK4_27130) | 26794..27237 | - | 444 | WP_016622506.1 | hypothetical protein | - |
| EFK4_RS13820 (EFK4_27140) | 27814..28179 | - | 366 | WP_236919508.1 | type II toxin-antitoxin system PemK/MazF family toxin | - |
| EFK4_RS13825 (EFK4_27150) | 28169..28414 | - | 246 | WP_016619114.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | - |
| EFK4_RS13830 (EFK4_27160) | 28421..28987 | - | 567 | WP_236919509.1 | hypothetical protein | - |
| EFK4_RS13835 (EFK4_27170) | 29564..30064 | - | 501 | WP_016632419.1 | hypothetical protein | - |
| EFK4_RS13840 (EFK4_27180) | 30078..30455 | - | 378 | WP_236919460.1 | hypothetical protein | - |
| EFK4_RS13845 (EFK4_27190) | 31160..31429 | + | 270 | WP_101732003.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| EFK4_RS13850 (EFK4_27200) | 31422..31679 | + | 258 | WP_016617334.1 | Txe/YoeB family addiction module toxin | Toxin |
| EFK4_RS13855 (EFK4_27210) | 32115..32591 | + | 477 | WP_229233467.1 | hypothetical protein | - |
| EFK4_RS13860 (EFK4_27220) | 32672..33136 | - | 465 | WP_236919461.1 | helix-turn-helix domain-containing protein | - |
| EFK4_RS13865 (EFK4_27230) | 33420..34040 | + | 621 | WP_236919462.1 | recombinase family protein | - |
| EFK4_RS13870 (EFK4_27240) | 34057..34341 | + | 285 | WP_236919463.1 | hypothetical protein | - |
| EFK4_RS13875 (EFK4_27250) | 34343..34549 | + | 207 | WP_236919464.1 | hypothetical protein | - |
| EFK4_RS13880 (EFK4_27260) | 34824..35024 | + | 201 | WP_236919465.1 | hypothetical protein | - |
| EFK4_RS13885 (EFK4_27270) | 35047..35295 | - | 249 | WP_050560578.1 | preprotein translocase subunit YajC | - |
| EFK4_RS13890 (EFK4_27280) | 35549..35779 | + | 231 | WP_010818068.1 | leucocin A/sakacin P family class II bacteriocin | - |
| EFK4_RS13895 (EFK4_27290) | 35769..36080 | + | 312 | WP_010818069.1 | hypothetical protein | - |
| EFK4_RS13900 (EFK4_27300) | 36218..36397 | + | 180 | WP_137274215.1 | class II bacteriocin | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..59411 | 59411 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 86 a.a. Molecular weight: 10192.51 Da Isoelectric Point: 8.4368
>T40228 WP_016617334.1 NZ_AP025272:31422-31679 [Enterococcus faecalis]
MIKAWSDDAWDDYLYWHDQGNKSNIKKINRLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYKVENETIFIYS
AKDHY
MIKAWSDDAWDDYLYWHDQGNKSNIKKINRLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYKVENETIFIYS
AKDHY
Download Length: 258 bp
>T40228 NZ_AP025272:31422-31679 [Enterococcus faecalis]
ATGATTAAAGCATGGTCTGATGATGCTTGGGATGACTATCTTTATTGGCATGATCAAGGAAACAAAAGCAACATAAAAAA
AATCAATAGGTTAATAAAAGATATAGATCGTTCGCCCTTTGCTGGATTAGGTAAACCCGAACCATTGAAGCATGATTTAT
CTGGGAAATGGTCTAGAAGAATTACAGATGAGCATAGGCTGATATATAAAGTTGAAAATGAAACGATATTCATTTATTCT
GCCAAAGATCATTATTAA
ATGATTAAAGCATGGTCTGATGATGCTTGGGATGACTATCTTTATTGGCATGATCAAGGAAACAAAAGCAACATAAAAAA
AATCAATAGGTTAATAAAAGATATAGATCGTTCGCCCTTTGCTGGATTAGGTAAACCCGAACCATTGAAGCATGATTTAT
CTGGGAAATGGTCTAGAAGAATTACAGATGAGCATAGGCTGATATATAAAGTTGAAAATGAAACGATATTCATTTATTCT
GCCAAAGATCATTATTAA
Antitoxin
Download Length: 90 a.a. Molecular weight: 10443.69 Da Isoelectric Point: 4.5732
>AT40228 WP_101732003.1 NZ_AP025272:31160-31429 [Enterococcus faecalis]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKEVEDTVVVLSKRDYDSMQETLRTLSNKYVMEKIRRGDEQFSKGLFKTH
DLIEVESDD
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKEVEDTVVVLSKRDYDSMQETLRTLSNKYVMEKIRRGDEQFSKGLFKTH
DLIEVESDD
Download Length: 270 bp
>AT40228 NZ_AP025272:31160-31429 [Enterococcus faecalis]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAACCTACGTAGTTATATGAAACAAGTTAATGAAGACGCTGAAACACT
TATTGTAACAAGTAAAGAAGTAGAAGATACAGTAGTGGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACTTTGA
GAACACTTAGTAATAAATATGTTATGGAAAAAATTCGTCGAGGAGATGAACAGTTTTCCAAAGGTTTATTTAAGACACAT
GATTTAATTGAGGTTGAATCTGATGATTAA
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAACCTACGTAGTTATATGAAACAAGTTAATGAAGACGCTGAAACACT
TATTGTAACAAGTAAAGAAGTAGAAGATACAGTAGTGGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACTTTGA
GAACACTTAGTAATAAATATGTTATGGAAAAAATTCGTCGAGGAGATGAACAGTTTTCCAAAGGTTTATTTAAGACACAT
GATTTAATTGAGGTTGAATCTGATGATTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|