40227

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 14551..15085 Replicon plasmid pEK4L
Accession NZ_AP025271
Organism Enterococcus faecalis strain K-4

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag EFK4_RS13430 Protein ID WP_002360769.1
Coordinates 14810..15085 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag EFK4_RS13425 Protein ID WP_002369771.1
Coordinates 14551..14817 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFK4_RS13385 (EFK4_26270) 9621..9815 - 195 WP_002367787.1 hypothetical protein -
EFK4_RS13390 (EFK4_26280) 9975..10196 - 222 WP_236919444.1 hypothetical protein -
EFK4_RS13395 (EFK4_26290) 10183..10467 - 285 WP_236919445.1 hypothetical protein -
EFK4_RS13400 (EFK4_26300) 10484..11104 - 621 WP_236919446.1 recombinase family protein -
EFK4_RS13405 (EFK4_26310) 11308..11538 + 231 WP_002362431.1 hypothetical protein -
EFK4_RS13410 (EFK4_26320) 11538..11879 + 342 WP_236919447.1 type II toxin-antitoxin system PemK/MazF family toxin -
EFK4_RS13415 (EFK4_26330) 11991..12929 + 939 WP_236919448.1 hypothetical protein -
EFK4_RS13420 (EFK4_26340) 13197..13799 + 603 WP_236919449.1 Fic family protein -
EFK4_RS13425 (EFK4_26350) 14551..14817 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EFK4_RS13430 (EFK4_26360) 14810..15085 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
EFK4_RS13435 (EFK4_26370) 15688..16707 - 1020 WP_236919450.1 IS30 family transposase -
EFK4_RS13440 (EFK4_26380) 17587..17913 - 327 WP_236919451.1 hypothetical protein -
EFK4_RS13445 17986..18099 - 114 Protein_22 IS6 family transposase -
EFK4_RS13450 (EFK4_26390) 18146..19291 - 1146 WP_236919452.1 dockerin type I domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - bsh 1..64595 64595


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-87)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T40227 WP_002360769.1 NZ_AP025271:14810-15085 [Enterococcus faecalis]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T40227 NZ_AP025271:14810-15085 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTACTAGTACTTTTACAAGAACAACAAACATTGCCACCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGCACACGAGAATGTCATATTGAACCAGACTGGTTGTTGATTTATAAAATTGATGGTGATAAATTAATTCTAACTTTA
GCTAGAATAGGCTCACATAGTGAATTATTCCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9804.15 Da        Isoelectric Point: 4.4791

>AT40227 WP_002369771.1 NZ_AP025271:14551-14817 [Enterococcus faecalis]
MATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT40227 NZ_AP025271:14551-14817 [Enterococcus faecalis]
TTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACTATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAAACTCTACAAGCATTAAAAGACATTAAAGAGGGAAACGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References