Detailed information of TA system TA040226

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 11308..11879
• Replicon: plasmid pEK4L
• Accession: NZ_AP025271
• Organism: Enterococcus faecalis strain K-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EFK4_RS13410
• Protein ID: WP_236919447.1
• Coordinates: 11538..11879 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: EFK4_RS13405
• Protein ID: WP_002362431.1
• Coordinates: 11308..11538 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFK4_RS13355 (EFK4_26220)	6341..6751	+	411	WP_130760244.1	hypothetical protein	-
EFK4_RS13360 (EFK4_26230)	6887..8203	-	1317	WP_236919441.1	Y-family DNA polymerase	-
EFK4_RS13365 (EFK4_26240)	8447..8707	-	261	WP_236919442.1	bacteriocin immunity protein	-
EFK4_RS13370 (EFK4_26250)	8732..9016	-	285	WP_236919443.1	bacteriocin immunity protein	-
EFK4_RS13375	9033..9248	-	216	Protein_8	class II bacteriocin	-
EFK4_RS13380 (EFK4_26260)	9418..9609	-	192	WP_002367788.1	hypothetical protein	-
EFK4_RS13385 (EFK4_26270)	9621..9815	-	195	WP_002367787.1	hypothetical protein	-
EFK4_RS13390 (EFK4_26280)	9975..10196	-	222	WP_236919444.1	hypothetical protein	-
EFK4_RS13395 (EFK4_26290)	10183..10467	-	285	WP_236919445.1	hypothetical protein	-
EFK4_RS13400 (EFK4_26300)	10484..11104	-	621	WP_236919446.1	recombinase family protein	-
EFK4_RS13405 (EFK4_26310)	11308..11538	+	231	WP_002362431.1	hypothetical protein	Antitoxin
EFK4_RS13410 (EFK4_26320)	11538..11879	+	342	WP_236919447.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EFK4_RS13415 (EFK4_26330)	11991..12929	+	939	WP_236919448.1	hypothetical protein	-
EFK4_RS13420 (EFK4_26340)	13197..13799	+	603	WP_236919449.1	Fic family protein	-
EFK4_RS13425 (EFK4_26350)	14551..14817	+	267	WP_002369771.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
EFK4_RS13430 (EFK4_26360)	14810..15085	+	276	WP_002360769.1	type II toxin-antitoxin system YafQ family toxin	-
EFK4_RS13435 (EFK4_26370)	15688..16707	-	1020	WP_236919450.1	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	bsh	1..64595	64595

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13226.44 Da
• Isoelectric Point: 8.8645

>T40226 WP_236919447.1 NZ_AP025271:11538-11879 [Enterococcus faecalis]
MSDETKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAIICPITSTIKNMPTRYTLPDEMETRGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T40226 NZ_AP025271:11538-11879 [Enterococcus faecalis]
ATGAGTGACGAAACAAAATATATTCCTAAAAAGGGTGATATTGTATGGATAGATTTCGATCCTTCAGCTGGAAAAGAAAT
ACAGAAGAGACGTCCAGGTCTAGTAGTTTCTAGATACGAATTTAATAGAAAGACAATGTTTGCAATAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCGTGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT40226 WP_002362431.1 NZ_AP025271:11308-11538 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT40226 NZ_AP025271:11308-11538 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAGGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGACGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Antitoxin

• AlphaFold DB: R3KHK9