Detailed information of TA system TA040221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2685538..2686109
• Replicon: chromosome
• Accession: NZ_AP025270
• Organism: Enterococcus faecalis strain K-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EFK4_RS12865
• Protein ID: WP_002354774.1
• Coordinates: 2685538..2685879 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: EFK4_RS12870
• Protein ID: WP_002354773.1
• Coordinates: 2685879..2686109 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EFK4_RS12860 (2681553)	2681553..2685167	-	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-
EFK4_RS12865 (2685538)	2685538..2685879	-	342	WP_002354774.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EFK4_RS12870 (2685879)	2685879..2686109	-	231	WP_002354773.1	hypothetical protein	Antitoxin
EFK4_RS12875 (2686283)	2686283..2686498	-	216	WP_002354768.1	zinc ribbon domain-containing protein	-
EFK4_RS12880 (2686637)	2686637..2687629	+	993	WP_002354765.1	biotin--[acetyl-CoA-carboxylase] ligase	-
EFK4_RS12885 (2687893)	2687893..2688522	-	630	WP_002354763.1	lytic polysaccharide monooxygenase	-
EFK4_RS12890 (2689215)	2689215..2690831	+	1617	WP_002381036.1	phosphatase PAP2/LCP family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13156.40 Da
• Isoelectric Point: 9.3984

>T40221 WP_002354774.1 NZ_AP025270:c2685879-2685538 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNIPTRYTLPDDIETQGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T40221 NZ_AP025270:c2685879-2685538 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT40221 WP_002354773.1 NZ_AP025270:c2686109-2685879 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT40221 NZ_AP025270:c2686109-2685879 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812