40210

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ImmA-IrrE/HTH_19(antitoxin)
Location 2323872..2324558 Replicon chromosome
Accession NZ_AP025270
Organism Enterococcus faecalis strain K-4

Toxin (Protein)


Gene name IrrE Uniprot ID -
Locus tag EFK4_RS11200 Protein ID WP_128722476.1
Coordinates 2324214..2324558 (+) Length 115 a.a.

Antitoxin (Protein)


Gene name ImmA Uniprot ID -
Locus tag EFK4_RS11195 Protein ID WP_010707137.1
Coordinates 2323872..2324195 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EFK4_RS11140 (2319045) 2319045..2320241 - 1197 WP_128722493.1 DEAD/DEAH box helicase -
EFK4_RS11145 (2320231) 2320231..2320524 - 294 WP_002418647.1 VRR-NUC domain-containing protein -
EFK4_RS11150 (2320527) 2320527..2321210 - 684 WP_128722477.1 DUF1642 domain-containing protein -
EFK4_RS11155 (2321322) 2321322..2321516 - 195 WP_010716255.1 hypothetical protein -
EFK4_RS11160 (2321517) 2321517..2321690 - 174 WP_010773396.1 hypothetical protein -
EFK4_RS11165 (2321696) 2321696..2321896 - 201 WP_002407633.1 hypothetical protein -
EFK4_RS11170 (2321934) 2321934..2322203 - 270 WP_002365131.1 hypothetical protein -
EFK4_RS11175 (2322279) 2322279..2322593 + 315 WP_002385638.1 hypothetical protein -
EFK4_RS14085 (2322574) 2322574..2322702 - 129 WP_002385637.1 hypothetical protein -
EFK4_RS11180 (2322718) 2322718..2322894 - 177 WP_002385636.1 helix-turn-helix transcriptional regulator -
EFK4_RS11185 (2322978) 2322978..2323361 + 384 WP_002385635.1 DUF2513 domain-containing protein -
EFK4_RS11190 (2323358) 2323358..2323567 - 210 WP_002415277.1 DUF2829 domain-containing protein -
EFK4_RS11195 (2323872) 2323872..2324195 + 324 WP_010707137.1 helix-turn-helix transcriptional regulator Antitoxin
EFK4_RS11200 (2324214) 2324214..2324558 + 345 WP_128722476.1 ImmA/IrrE family metallo-endopeptidase Toxin
EFK4_RS11205 (2324648) 2324648..2325412 + 765 WP_236919323.1 LysM domain-containing protein -
EFK4_RS11210 (2325506) 2325506..2326153 - 648 WP_236919324.1 hypothetical protein -
EFK4_RS11215 (2326339) 2326339..2327520 + 1182 WP_207463243.1 site-specific integrase -
EFK4_RS11220 (2327623) 2327623..2327772 - 150 WP_002356321.1 50S ribosomal protein L33 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2292020..2327520 35500


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

IrrE N-terminal-like domain

(22-86)

Antitoxin

Cro/C1-type helix-turn-helix domain

(5-59)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 13670.65 Da        Isoelectric Point: 5.6183

>T40210 WP_128722476.1 NZ_AP025270:2324214-2324558 [Enterococcus faecalis]
MKSIKELVEEYNVELVFTTLNKRAYFDPTYGIIFVNQNLTPSEQEEAIYHELKHVKDHVDIMALYKIPVFRSKMESEAEQ
YMFRSLIEKYEGQYNYSNVIAHYNLKMGQEIYLK

Download         Length: 345 bp

>T40210 NZ_AP025270:2324214-2324558 [Enterococcus faecalis]
ATGAAAAGTATCAAAGAGTTGGTAGAAGAATATAATGTGGAGTTAGTTTTTACTACTTTAAACAAACGCGCATATTTCGA
TCCTACCTACGGTATCATATTTGTAAATCAAAATTTAACACCATCAGAACAAGAAGAAGCAATATATCACGAATTAAAGC
ATGTAAAAGACCATGTGGATATAATGGCATTGTATAAAATTCCTGTTTTTCGTTCTAAGATGGAATCCGAAGCAGAACAA
TATATGTTTAGAAGCTTAATCGAAAAATATGAAGGACAATACAATTACTCAAATGTTATAGCTCATTACAACTTAAAAAT
GGGACAAGAAATTTATTTGAAATAA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12278.84 Da        Isoelectric Point: 5.2423

>AT40210 WP_010707137.1 NZ_AP025270:2323872-2324195 [Enterococcus faecalis]
MTVFDRVKKLADSQKISIVELEEKLNFSRNSLYAWKKSKPSIDKLEAVANYFGVSTDYLLGREVSNKSKQSDDLDDVLDN
VMSFDGEPLDDHDREVIRAYLKGRFGK

Download         Length: 324 bp

>AT40210 NZ_AP025270:2323872-2324195 [Enterococcus faecalis]
ATGACTGTATTTGATAGAGTGAAAAAATTAGCAGATAGTCAGAAAATATCTATTGTCGAACTTGAAGAAAAGTTAAATTT
CAGTCGAAATTCATTATACGCTTGGAAAAAAAGTAAACCTTCTATTGATAAATTAGAAGCTGTTGCAAATTATTTTGGAG
TTTCAACGGATTATTTATTAGGTCGTGAAGTTTCTAATAAATCAAAGCAATCTGATGATTTAGATGATGTACTGGATAAC
GTCATGAGTTTTGACGGTGAACCGCTTGATGATCATGACAGAGAAGTTATTCGTGCATATTTAAAGGGTAGATTCGGGAA
ATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References