Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 542287..542923 Replicon chromosome
Accession NZ_AP025264
Organism Bacillus altitudinis strain NC3

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag NC3_RS02585 Protein ID WP_003214169.1
Coordinates 542573..542923 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag NC3_RS02580 Protein ID WP_003214273.1
Coordinates 542287..542568 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NC3_RS02560 (NC3_04770) 538441..539046 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
NC3_RS02565 (NC3_04780) 539141..539506 + 366 WP_017358398.1 holo-ACP synthase -
NC3_RS02570 (NC3_04790) 539667..540683 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
NC3_RS02575 (NC3_04800) 540812..541993 + 1182 WP_165378700.1 alanine racemase -
NC3_RS02580 (NC3_04810) 542287..542568 + 282 WP_003214273.1 hypothetical protein Antitoxin
NC3_RS02585 (NC3_04820) 542573..542923 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
NC3_RS02590 (NC3_04830) 543038..543868 + 831 WP_017358395.1 RsbT co-antagonist protein RsbRA -
NC3_RS02595 (NC3_04840) 543873..544241 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
NC3_RS02600 (NC3_04850) 544244..544645 + 402 WP_003214085.1 anti-sigma regulatory factor -
NC3_RS02605 (NC3_04860) 544656..545663 + 1008 WP_074041673.1 PP2C family protein-serine/threonine phosphatase -
NC3_RS02610 (NC3_04870) 545723..546052 + 330 WP_008346902.1 anti-sigma factor antagonist -
NC3_RS02615 (NC3_04880) 546049..546537 + 489 WP_007496471.1 anti-sigma B factor RsbW -
NC3_RS02620 (NC3_04890) 546503..547291 + 789 WP_025206796.1 RNA polymerase sigma factor SigB -
NC3_RS02625 (NC3_04900) 547291..547890 + 600 WP_008346897.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T40206 WP_003214169.1 NZ_AP025264:542573-542923 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T40206 NZ_AP025264:542573-542923 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCGGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGCTACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT40206 WP_003214273.1 NZ_AP025264:542287-542568 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT40206 NZ_AP025264:542287-542568 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References