Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 533228..533864 Replicon chromosome
Accession NZ_AP025262
Organism Bacillus altitudinis strain TM2

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag TM2_RS02585 Protein ID WP_003214169.1
Coordinates 533514..533864 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag TM2_RS02580 Protein ID WP_003214273.1
Coordinates 533228..533509 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
TM2_RS02560 (TM2_04720) 529382..529987 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
TM2_RS02565 (TM2_04730) 530082..530447 + 366 WP_007496490.1 holo-ACP synthase -
TM2_RS02570 (TM2_04740) 530608..531624 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
TM2_RS02575 (TM2_04750) 531753..532934 + 1182 WP_236810238.1 alanine racemase -
TM2_RS02580 (TM2_04760) 533228..533509 + 282 WP_003214273.1 hypothetical protein Antitoxin
TM2_RS02585 (TM2_04770) 533514..533864 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
TM2_RS02590 (TM2_04780) 533981..534811 + 831 WP_008346908.1 RsbT co-antagonist protein RsbRA -
TM2_RS02595 (TM2_04790) 534816..535184 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
TM2_RS02600 (TM2_04800) 535187..535588 + 402 WP_003214085.1 anti-sigma regulatory factor -
TM2_RS02605 (TM2_04810) 535599..536606 + 1008 WP_008346904.1 PP2C family protein-serine/threonine phosphatase -
TM2_RS02610 (TM2_04820) 536666..536995 + 330 WP_236810239.1 anti-sigma factor antagonist -
TM2_RS02615 (TM2_04830) 536992..537480 + 489 WP_007496471.1 anti-sigma B factor RsbW -
TM2_RS02620 (TM2_04840) 537446..538234 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
TM2_RS02625 (TM2_04850) 538234..538833 + 600 WP_017358391.1 PP2C family serine/threonine-protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T40205 WP_003214169.1 NZ_AP025262:533514-533864 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T40205 NZ_AP025262:533514-533864 [Bacillus altitudinis]
TTGATTGTTAAACGCGGTGATGTTTACTTTGCTGATTTATCTCCTGTTGTTGGCTCCGAGCAGGGCGGGGTTCGCCCAGT
ATTGGTGATTCAAAATGACATTGGAAATCGCTTCAGCCCGACTGCTATCGTTGCAGCCATAACAGCCCAAATACAAAAAG
CGAAATTACCGACTCACGTCGAAATTAATGCAAAGCGATACGGCTTTGAACGAGATTCTGTCATTCTTTTGGAACAGATT
CGTACGATCGATAAACAAAGATTGACAGATAAAATCACACATCTTGATGATGAGATGATGGAGAAGGTTGATGAAGCCTT
ACAAGTTAGTTTGGCTCTTATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT40205 WP_003214273.1 NZ_AP025262:533228-533509 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT40205 NZ_AP025262:533228-533509 [Bacillus altitudinis]
TTGTCTGAATCCAGCGCAACAAACGAAATGAAGATTCGACTGCCGGAGGCTTTAATGTCTGAGATCGAACGAGTAGCAGA
GCAGGATAAGGTAACAAGAAATGAATTGATCTACCGAGCGACAAAAACGTATATTCGTGAACGTAATATTCGACAAAATC
GTGAATCGATGAGACGCGGCTACATGGAGATGGCGAAAATTAATCTGAATATCTCTTCAGAGGCACAATACGCAGAATTT
GAGGCTGAGAATACAGTTGAGCGATTAGTCAGCGGGGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References