Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 61545..62455 Replicon plasmid pOCUhvKP1
Accession NZ_AP025247
Organism Klebsiella pneumoniae strain OCU_hvKP1

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag L6440_RS26400 Protein ID WP_004026354.1
Coordinates 61985..62455 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag L6440_RS26395 Protein ID WP_004026357.1
Coordinates 61545..61988 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6440_RS26365 (OCUBac04_50850) 56563..57084 + 522 WP_004213635.1 ferric citrate uptake sigma factor FecI -
L6440_RS26370 57081..58034 + 954 Protein_56 fec operon regulator FecR -
L6440_RS26375 (OCUBac04_50880) 58121..60247 + 2127 WP_004213640.1 TonB-dependent Fe(3+) dicitrate receptor FecA -
L6440_RS26380 60322..60447 + 126 Protein_58 Fe3+-citrate ABC transporter substrate-binding protein -
L6440_RS26385 (OCUBac04_50890) 60622..60843 + 222 WP_004213643.1 DUF2188 domain-containing protein -
L6440_RS26390 60941..61444 + 504 Protein_60 DUF4113 domain-containing protein -
L6440_RS26395 (OCUBac04_50900) 61545..61988 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
L6440_RS26400 (OCUBac04_50910) 61985..62455 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
L6440_RS26405 62604..63301 + 698 WP_223175001.1 IS1-like element IS1A family transposase -
L6440_RS26410 (OCUBac04_50940) 64687..65337 - 651 WP_068893702.1 DUF1173 family protein -
L6440_RS26415 (OCUBac04_50950) 65370..65636 - 267 WP_223175074.1 DUF1173 family protein -
L6440_RS26420 (OCUBac04_50960) 65814..66308 + 495 WP_004212794.1 thermonuclease family protein -
L6440_RS26425 (OCUBac04_50970) 66645..67043 + 399 WP_004212796.1 H-NS family nucleoid-associated regulatory protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - rmpA / iroN / iroD / iroC / iroB / iucA / iucB / iucC / iucD / iutA / rmpA 1..215041 215041
- flank IS/Tn - - 62798..63301 503


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T40193 WP_004026354.1 NZ_AP025247:61985-62455 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T40193 NZ_AP025247:61985-62455 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT40193 WP_004026357.1 NZ_AP025247:61545-61988 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT40193 NZ_AP025247:61545-61988 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References