Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5357310..5357935 Replicon chromosome
Accession NZ_AP025246
Organism Klebsiella pneumoniae strain OCU_hvKP1

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag L6440_RS25730 Protein ID WP_012737091.1
Coordinates 5357310..5357693 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag L6440_RS25735 Protein ID WP_004150355.1
Coordinates 5357693..5357935 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6440_RS25715 (5354676) 5354676..5355578 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
L6440_RS25720 (5355575) 5355575..5356210 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
L6440_RS25725 (5356207) 5356207..5357136 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
L6440_RS25730 (5357310) 5357310..5357693 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
L6440_RS25735 (5357693) 5357693..5357935 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
L6440_RS25740 (5358140) 5358140..5359057 + 918 WP_012737090.1 alpha/beta hydrolase -
L6440_RS25745 (5359071) 5359071..5360012 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
L6440_RS25750 (5360057) 5360057..5360494 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
L6440_RS25755 (5360491) 5360491..5361351 - 861 WP_004146232.1 virulence factor BrkB family protein -
L6440_RS25760 (5361345) 5361345..5361944 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T40192 WP_012737091.1 NZ_AP025246:c5357693-5357310 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T40192 NZ_AP025246:c5357693-5357310 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT40192 WP_004150355.1 NZ_AP025246:c5357935-5357693 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT40192 NZ_AP025246:c5357935-5357693 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References