Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4075219..4075838 Replicon chromosome
Accession NZ_AP025246
Organism Klebsiella pneumoniae strain OCU_hvKP1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag L6440_RS19760 Protein ID WP_002892050.1
Coordinates 4075219..4075437 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag L6440_RS19765 Protein ID WP_002892066.1
Coordinates 4075464..4075838 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6440_RS19725 (4071266) 4071266..4071529 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
L6440_RS19730 (4071529) 4071529..4071669 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
L6440_RS19735 (4071666) 4071666..4072364 - 699 WP_002892021.1 GNAT family protein -
L6440_RS19740 (4072465) 4072465..4073916 + 1452 WP_014907971.1 PLP-dependent aminotransferase family protein -
L6440_RS19745 (4073891) 4073891..4074361 - 471 WP_002892026.1 YlaC family protein -
L6440_RS19750 (4074382) 4074382..4074522 + 141 WP_004147370.1 hypothetical protein -
L6440_RS19755 (4074494) 4074494..4075060 - 567 WP_002892030.1 maltose O-acetyltransferase -
L6440_RS19760 (4075219) 4075219..4075437 - 219 WP_002892050.1 HHA domain-containing protein Toxin
L6440_RS19765 (4075464) 4075464..4075838 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
L6440_RS19770 (4076324) 4076324..4079470 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
L6440_RS19775 (4079493) 4079493..4080686 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40189 WP_002892050.1 NZ_AP025246:c4075437-4075219 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T40189 NZ_AP025246:c4075437-4075219 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT40189 WP_002892066.1 NZ_AP025246:c4075838-4075464 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT40189 NZ_AP025246:c4075838-4075464 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References