Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | mazEF/MazF(toxin) |
| Location | 458108..458744 | Replicon | chromosome |
| Accession | NZ_AP025224 | ||
| Organism | Bacillus subtilis subsp. natto strain G8 | ||
Toxin (Protein)
| Gene name | mazF | Uniprot ID | G4NU33 |
| Locus tag | BSG8_RS02380 | Protein ID | WP_003156187.1 |
| Coordinates | 458394..458744 (+) | Length | 117 a.a. |
Antitoxin (Protein)
| Gene name | mazE | Uniprot ID | G4NU32 |
| Locus tag | BSG8_RS02375 | Protein ID | WP_003225183.1 |
| Coordinates | 458108..458389 (+) | Length | 94 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| BSG8_RS02355 (BSG8_04670) | 454467..455066 | - | 600 | WP_014478896.1 | rhomboid family intramembrane serine protease | - |
| BSG8_RS02360 (BSG8_04680) | 455161..455526 | + | 366 | WP_014478897.1 | holo-ACP synthase | - |
| BSG8_RS02365 (BSG8_04690) | 455692..456708 | + | 1017 | WP_003234282.1 | outer membrane lipoprotein carrier protein LolA | - |
| BSG8_RS02370 (BSG8_04700) | 456823..457992 | + | 1170 | WP_014478898.1 | alanine racemase | - |
| BSG8_RS02375 (BSG8_04710) | 458108..458389 | + | 282 | WP_003225183.1 | type II toxin-antitoxin system antitoxin EndoAI | Antitoxin |
| BSG8_RS02380 (BSG8_04720) | 458394..458744 | + | 351 | WP_003156187.1 | type II toxin-antitoxin system endoribonuclease NdoA | Toxin |
| BSG8_RS02385 (BSG8_04730) | 458860..459684 | + | 825 | WP_009966610.1 | RsbT co-antagonist protein RsbRA | - |
| BSG8_RS02390 (BSG8_04740) | 459689..460054 | + | 366 | WP_003225190.1 | RsbT antagonist protein RsbS | - |
| BSG8_RS02395 (BSG8_04750) | 460058..460459 | + | 402 | WP_014478899.1 | serine/threonine-protein kinase RsbT | - |
| BSG8_RS02400 (BSG8_04760) | 460471..461478 | + | 1008 | WP_014478900.1 | phosphoserine phosphatase RsbU | - |
| BSG8_RS02405 (BSG8_04770) | 461547..461876 | + | 330 | WP_003234298.1 | anti-sigma factor antagonist RsbV | - |
| BSG8_RS02410 (BSG8_04780) | 461873..462355 | + | 483 | WP_003234299.1 | anti-sigma B factor RsbW | - |
| BSG8_RS02415 (BSG8_04790) | 462321..463109 | + | 789 | WP_003246715.1 | RNA polymerase sigma factor SigB | - |
| BSG8_RS02420 (BSG8_04800) | 463109..463708 | + | 600 | WP_003246608.1 | phosphoserine phosphatase RsbX | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 117 a.a. Molecular weight: 12977.98 Da Isoelectric Point: 4.8781
>T40165 WP_003156187.1 NZ_AP025224:458394-458744 [Bacillus subtilis subsp. natto]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF
Download Length: 351 bp
>T40165 NZ_AP025224:458394-458744 [Bacillus subtilis subsp. natto]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
CTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG
Antitoxin
Download Length: 94 a.a. Molecular weight: 10546.92 Da Isoelectric Point: 6.4708
>AT40165 WP_003225183.1 NZ_AP025224:458108-458389 [Bacillus subtilis subsp. natto]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG
Download Length: 282 bp
>AT40165 NZ_AP025224:458108-458389 [Bacillus subtilis subsp. natto]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCATATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|