Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 93376..93977 Replicon plasmid p20171101CC-1
Accession NZ_AP025221
Organism Escherichia coli strain 2017.11.01CC

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LPH31_RS23365 Protein ID WP_001216034.1
Coordinates 93376..93756 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LPH31_RS23370 Protein ID WP_001190712.1
Coordinates 93756..93977 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH31_RS23335 (VEGS20_45360) 90607..90816 + 210 WP_072663797.1 c1 repressor inactivator -
LPH31_RS23340 (VEGS20_45370) 90927..91778 + 852 WP_000611664.1 phage repressor protein C1 -
LPH31_RS23345 91811..92143 - 333 WP_224205061.1 phage antirepressor KilAC domain-containing protein -
LPH31_RS23350 92264..92389 - 126 Protein_91 phage antirepressor Ant -
LPH31_RS23360 (VEGS20_45410) 93192..93371 - 180 WP_038997082.1 hypothetical protein -
LPH31_RS23365 (VEGS20_45420) 93376..93756 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LPH31_RS23370 (VEGS20_45430) 93756..93977 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LPH31_RS23375 (VEGS20_45440) 94160..95716 + 1557 WP_038997084.1 type I restriction-modification system subunit M -
LPH31_RS23380 (VEGS20_45450) 95713..96978 + 1266 WP_106693635.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / blaTEM-1B - 1..129419 129419


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T40164 WP_001216034.1 NZ_AP025221:c93756-93376 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T40164 NZ_AP025221:c93756-93376 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT40164 WP_001190712.1 NZ_AP025221:c93977-93756 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT40164 NZ_AP025221:c93977-93756 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References