Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4248721..4249218 Replicon chromosome
Accession NZ_AP025220
Organism Escherichia coli strain 2017.11.01CC

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LPH31_RS20605 Protein ID WP_001173343.1
Coordinates 4248721..4248894 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A2U9LI53
Locus tag LPH31_RS20610 Protein ID WP_071597404.1
Coordinates 4248922..4249218 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH31_RS20595 (4245386) 4245386..4246843 + 1458 WP_072778207.1 dipeptide/tripeptide permease DtpC -
LPH31_RS20600 (4247080) 4247080..4248597 + 1518 WP_001295074.1 lysine--tRNA ligase -
LPH31_RS20605 (4248721) 4248721..4248894 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LPH31_RS20610 (4248922) 4248922..4249218 - 297 WP_071597404.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LPH31_RS20615 (4249445) 4249445..4249717 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LPH31_RS20620 (4249729) 4249729..4249959 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
LPH31_RS20625 (4250140) 4250140..4251771 + 1632 WP_001565376.1 sensor histidine kinase -
LPH31_RS20630 (4251768) 4251768..4252487 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(6-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40162 WP_001173343.1 NZ_AP025220:c4248894-4248721 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40162 NZ_AP025220:c4248894-4248721 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11495.99 Da        Isoelectric Point: 4.1705

>AT40162 WP_071597404.1 NZ_AP025220:c4249218-4248922 [Escherichia coli]
MEGKNRFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40162 NZ_AP025220:c4249218-4248922 [Escherichia coli]
ATGGAAGGTAAAAACAGGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A2U9LI53

References