Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3469104..3469722 Replicon chromosome
Accession NZ_AP025220
Organism Escherichia coli strain 2017.11.01CC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LPH31_RS16930 Protein ID WP_001291435.1
Coordinates 3469504..3469722 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LPH31_RS16925 Protein ID WP_000344800.1
Coordinates 3469104..3469478 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LPH31_RS16915 (3464193) 3464193..3465386 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LPH31_RS16920 (3465409) 3465409..3468558 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LPH31_RS16925 (3469104) 3469104..3469478 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LPH31_RS16930 (3469504) 3469504..3469722 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LPH31_RS16935 (3469894) 3469894..3470445 + 552 WP_000102564.1 maltose O-acetyltransferase -
LPH31_RS16940 (3470561) 3470561..3471031 + 471 WP_000136192.1 YlaC family protein -
LPH31_RS16945 (3471195) 3471195..3472745 + 1551 WP_229430189.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LPH31_RS16950 (3472787) 3472787..3473140 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LPH31_RS16960 (3473519) 3473519..3473830 + 312 WP_000409911.1 MGMT family protein -
LPH31_RS16965 (3473861) 3473861..3474433 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40160 WP_001291435.1 NZ_AP025220:3469504-3469722 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40160 NZ_AP025220:3469504-3469722 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40160 WP_000344800.1 NZ_AP025220:3469104-3469478 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40160 NZ_AP025220:3469104-3469478 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References